HTS031M - ChemiSCREEN™ CRTH2 / DP2 Prostanoid Receptor Membrane Preparation

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    Chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) is a GPCR related to FPR-like chemoattractant receptors that is highly expressed on Th2 cells, eosinophils, basophils, and type 2 cytotoxic T cells (Nagata et al., 1999).  The most potent ligand for CRTH2 is prostaglandin D2 (PGD2), a major mast cell-derived allergic mediator (Hirai et al., 2001).  Another GPCR, DP, is activated by PGD2, and the relative contributions of DP and CRTH2 to PGD2-mediated allergic disease are being elucidated.  A small molecule antagonist of CRTH2, ramatroban, attenuates PGD2-mediated bronchial hyperresponsiveness and antigen-induced inflammation (Sugimoto et al., 2003;  Shichijo et al., 2003). The cloned human CRTH2 expressing cell line is made in the Chem-5 host, which supports high levels of CRTH2 expression on the cell surface and contains high levels of the promiscuous G proteins to enhance coupling of the receptor to the calcium-signaling pathway. The cell line is an ideal tool for screening antagonists of interaction between CRTH2 and its ligand.

    Additional Resource :  HTS031M100614 Datasheet

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial
    Applications: Radioligand Binding Assay, GTPγS Binding
    Species: Human CRTH2 (Accession number AB008535)
    Host Cell: Chem-5, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Reference 1: 1. Hirai, H. et al. (2001) Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2. J. Exp. Med. 193:255-261.
    Reference 2: 2. Nagata, K. et al. (1999) Selective expression of a novel surface molecule by human Th2 cells in vivo. J. Immunol. 162:1278-1286.
    Reference 3: 3. Shichijo M. et al. (2003) Chemoattractant receptor-homologous molecule expressed on Th2 cells activation in vivo increases blood leukocyte counts and its blockade abrogates 13:14-dihydro-15-keto-prostaglandin D2-induced eosinophilia in rats. J. P