Calcium Flux Assay

HTS030RTA - Ready-to-Assay™ PTH1 Parathyroid Hormone Receptor Frozen Cells

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HTS030RTA
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  • Product Description

    Parathyroid hormone (PTH) plays a critical role in mineral ion homeostasis, particularly in bone and kidney (Mannstadt et al., 1999; Gensure et al., 2005).  A related peptide, parathyroid hormone-related peptide (PTHrP), plays an important role in skeletal development.  Both peptides exert their biological actions by binding to a class B GPCR, PTH1, that signals primarily by activation of adenylate cyclase (Schipani et al., 1993).  Mutations in PTH1 have been identified as the cause of Jansen's metaphyseal chondrodysplasia, Blomstrand's chondrodysplasia, and enchondromatosis (Schipani and Provot, 2003).  Intermittent treatment with PTH has important clinical utility in building bone mass in patients with osteoporosis (Rosen, 2003).  Cloned human PTH1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for antagonists of interactions between PTH1 and its ligands.

    Additional Resource: HTS030RTA092714_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay, cAMP Accumulation
    Entrez Gene Number: NM_000316
    Protein Targets: PTH1
    Target Sub-family: Parathyroid
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: PTH1 cDNA (Accession Number: NM_000316; see CODING SEQUENCE below) expressed from a proprietary E5 promoter plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Gensure RC et al. (2005) Parathyroid hormone and parathyroid hormone-related peptide, and their receptors. Biochem. Biophys. Res. Commun. 328: 666-78.
    Reference 2: 2. Mannstadt M et al. (1999) Receptors for PTH and PTHrP: their biological importance and functional properties. Am. J. Physiol. 277(5 Pt 2): F665-75.
    Reference 3: 3. Rosen CJ (2003) The cellular and clinical parameters of anabolic therapy for osteoporosis. Crit. Rev. Eukaryot. Gene Expr. 13: 25-38.
    Reference 4: 3. Rosen CJ (2003) The cellular and clinical parameters of anabolic therapy for osteoporosis. Crit. Rev. Eukaryot. Gene Expr. 13: 25-38.
    Reference 5: 5. Schipani E (1993) Identical complementary deoxyribonucleic acids encode a human renal and bone parathyroid hormone (PTH)/PTH-related peptide receptor. Endocrinology 132: 2157-65.
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