HTS030C - ChemiSCREEN™ Human Recombinant PTH1 Parathyroid Hormone Receptor Calcium-Optimized Stable Cell Line

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    Parathyroid hormone (PTH) plays a critical role in mineral ion homeostasis, particularly in bone and kidney (Mannstadt et al., 1999; Gensure et al., 2005).  A related peptide, parathyroid hormone-related peptide (PTHrP), plays an important role in skeletal development.  Both peptides exert their biological actions by binding to a class B GPCR, PTH1, that signals primarily by activation of adenylate cyclase (Schipani et al., 1993).  Mutations in PTH1 have been identified as the cause of Jansen's metaphyseal chondrodysplasia, Blomstrand's chondrodysplasia, and enchondromatosis (Schipani and Provot, 2003).  Intermittent treatment with PTH has important clinical utility in building bone mass in patients with osteoporosis (Rosen, 2003).  The cloned human PTH1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for antagonists of interactions between PTH1 and its ligands.

    Additional Resource :  HTS030C060315 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human PTH1 cDNA (Accession Number: NM_000316) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Gensure RC et al. (2005) Parathyroid hormone and parathyroid hormone-related peptide, and their receptors. Biochem. Biophys. Res. Commun. 328: 666-78.
    Reference 2: 2. Mannstadt M et al. (1999) Receptors for PTH and PTHrP: their biological importance and functional properties. Am. J. Physiol. 277(5 Pt 2): F665-75.
    Reference 3: 3. Rosen CJ (2003) The cellular and clinical parameters of anabolic therapy for osteoporosis. Crit. Rev. Eukaryot. Gene Expr. 13: 25-38.
    Reference 4: 4. Schipani E and Provot S. (2003) PTHrP, PTH, and the PTH/PTHrP receptor in endochondral bone development. Birth Defects Res. C. Embryo Today 69: 352-62.
    Reference 5: 5. Schipani E (1993) Identical complementary deoxyribonucleic acids encode a human renal and bone parathyroid hormone (PTH)/PTH-related peptide receptor. Endocrinology 132: 2157-65.