Calcium Flux Assay

HTS026RTA - Ready-to-Assay™ MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells

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    Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992).  When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001).  Two G protein-coupled receptors for MCH, MCH1 and MCH2, have been identified in humans, although rodents appear only to have MCH1 (Tan et al., 2002).  Both MCH1 and MCH2 are expressed primarily in brain (An et al., 2001).  MCH1 has been demonstrated by genetic and pharmacological methods in rodent models to mediate obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002; Segal-Lieberman, et al., 2003;  Marsh et al., 2002).  However, MCH2 might play a similar role in the species that express it. Cloned human MCH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCH2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MCH2.

    Additional Resource: HTS026RTA092714_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: AY562946
    Protein Targets: MCH2
    Target Sub-family: Melanin
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: Human MCHR2 cDNA (Accession Number: AY562946) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. An S. et al. (2001) Identification and characterization of a melanin-concentrating hormone receptor. Proc. Natl. Acad. Sci. USA 98: 7576-7581.
    Reference 2: 2. Bittencourt J.C., et al. (1992) The melanin-concentrating hormone system of the rat brain: an immuno- and hybridization histochemical characterization. J. Comp. Neurol. 319: 218-45.
    Reference 3: 3. Borowsky B., et al. (2002) Antidepressant, anxiolytic and anorectic effects of a melanin-concentrating hormone-1 receptor antagonist. Nat. Med. 8: 825-30.
    Reference 4: 4. Ludwig D.S., et al. (2001) Melanin-concentrating hormone overexpression in transgenic mice leads to obesity and insulin resistance. J. Clin. Invest. 107: 379-86.
    Reference 5: 5. Marsh D.J., et al. (2002) Melanin-concentrating hormone 1 receptor-deficient mice are lean, hyperactive, and hyperphagic and have altered metabolism. Proc. Natl. Acad. Sci. USA 99: 3240-5.