Calcium Flux Assay

HTS018RTA - Ready-to-Assay™ FPR1 N-formylpeptide Receptor Frozen Cells

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  • Product Description

    FPR1 is a Gi-coupled GPCR that binds and is activated by short, N-formyl peptides, such as N-Formyl-Met-Leu-Phe (fMLF).  N-formyl peptides are typically derived from bacteria and mediate host recruitment of leukocytes, which highly express FPR1, to sites of bacterial infection (Gao et al., 1999).  In addition, FPR1 is expressed in the vasculature, secretory epithelial cells, neurons and other tissues (Becker et al., 1998).  Other ligands, such as mitochondrially encoded formyl peptides, amyloid proteins, and virus-derived peptides, have also been found to activate FPR1 and/or related GPCRs and may be important in the pathophysiology of amyloidoses, Alzheimer’s disease and HIV (Le et al., 2002).  Cloned human FPR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FPR1expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at FPR1 Receptor.

    Additional Resource: HTS018RTA092614_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: M60626
    Protein Targets: FPR1
    Target Sub-family: N-formylpeptide
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: FPR1 cDNA (Accession Number: M60626; see CODING SEQUENCE below) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Becker EL et al. (1998) Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells. Cell Tissue Res. 292: 129-35.
    Reference 2: 2. Gao JL et al. (1999) Impaired antibacterial host defense in mice lacking the N-formylpeptide receptor. J. Exp. Med. 189: 657-62
    Reference 3: 3. Le Y et al. (2002) Formyl-peptide receptors revisited. Trends Immunol. 23: 541-8.