Calcium Flux Assay

HTS018C - ChemiSCREEN™ Human Recombinant FPR1 N-formyl Peptide Receptor Calcium-Optimized Stable Cell Line

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  • Product Description

    FPR1 is a Gi-coupled GPCR that binds and is activated by short, N-formyl peptides, such as N-Formyl-Met-Leu-Phe (fMLF).  N-formyl peptides are typically derived from bacteria and mediate host recruitment of leukocytes, which highly express FPR1, to sites of bacterial infection (Gao et al., 1999).  In addition, FPR1 is expressed in the vasculature, secretory epithelial cells, neurons and other tissues  (Becker et al., 1998).  Other ligands, such as mitochondrially encoded formyl peptides, amyloid proteins, and virus-derived peptides, have also been found to activate FPR1 and/or related GPCRs and may be important in the pathophysiology of amyloidoses, Alzheimer’s disease and HIV (Le et al., 2002).  The cloned human FPR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FPR1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for antagonists of interactions between FPR1 and its ligands.

    Additional Resource :  HTS018C050815 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human FPR1 cDNA (Accession Number: M60626- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Becker EL et al. (1998) Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells. Cell Tissue Res. 292: 129-35.
    Reference 2: 2. Gao JL et al. (1999) Impaired antibacterial host defense in mice lacking the N-formylpeptide receptor. J. Exp. Med. 189: 657-62.
    Reference 3: 3. Le Y et al. (2002) Formyl-peptide receptors revisited. Trends Immunol. 23: 541-8.