Calcium Flux Assay

HTS015RTA - Ready-to-Assay™ CX3CR1 Chemokine Receptor Frozen Cells

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    CX3CR1 is a GPCR expressed on natural killer cells, cytotoxic T lymphocytes, and macrophages.  The sole ligand for CX3CR1, fractalkine, is an unusual chemokine that is expressed as a transmembrane molecule with a CX3C domain and a mucin domain (Imai et al., 1997).  Fractalkine is highly expressed on endothelial cells activated by TNFα and other proinflammatory cytokines, and fractalkine/CX3CR1 interactions mediate recruitment of macrophages into the atherosclerotic plaque (Lesnick et al., 2003; McDermott et al., 2003).  In addition, fractalkine and CX3CR1 have been implicated in the pathogenesis of glomerulonephritis, HIV infection and rheumatoid arthritis (Ito et al., 2002; Faure et al., 2003; Nanki et al., 2002). Cloned human CX3CR1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CX3CR1 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CX3CR1.

    Additional Resource: HTS015RTA080914_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: U28934
    Protein Targets: CX3CR1
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-4, an adherent rat hematopoietic cell line expressing endogenous Ga15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exogenous Gene Expression: CX3CR1cDNA (Accession Number: U28934; see CODING SEQUENCE below) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Faure S. et al. (2003) Deleterious genetic influence of CX3CR1 genotypes on HIV-1 disease progression. J Acquir. Immune Defic. Syndr. 32: 335-7
    Reference 2: 2. Imai T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91: 521-30.
    Reference 3: 3. Ito Y. et al. (2002) Fractalkine expression and the recruitment of CX3CR1+ cells in the prolonged mesangial proliferative glomerulonephritis. Kidney Int. 61: 2044-57.
    Reference 4: 4. Lesnick P. et al. (2003) Decreased atherosclerosis in CX3CR1-/- mice reveals a role for fractalkine in atherogenesis. J. Clin. Invest. 111: 333-40.
    Reference 5: 5. McDermott D.H. et al. (2003) Chemokine receptor mutant CX3CR1-M280 has impaired adhesive function and correlates with protection from cardiovascular disease in humans. J. Clin. Invest. 111: 1241-50.
    Reference 6: 6. Nanki T. et al. (2002) Migration of CX3CR1-positive T cells producing type 1 cytokines and cytotoxic molecules into the synovium of patients with rheumatoid arthritis. Arthritis Rheum. 46: 2878-83.