Calcium Flux Assay

HTS014C - ChemiSCREEN™ Human Recombinant CCR10 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    CCR10, previously known as GPR2, is a GPCR that is expressed in dermal endothelial cells, fibroblasts, melanocytes, and circulating T cells and PBMCs (Homey et al., 2000). Two chemokines, CTACK/CCL27 and MEC/CCL28, bind to CCR10 and stimulate migration and calcium flux (Pan et al., 2000; Wang et al., 2000). Expression of CTACK by keratinocytes, and CCR10 by lymphocytes from skin of patients with psoriasis and dermatitis, implicates this receptor ligand pair in skin inflammation. In addition, disruption of CCL27-CCR10 interaction reduces skin inflammation induced by allergen (Homey et al., 2002). CCR10 expressed on IgA antibody-secreting cells also mediates their migration to CCL28 expressed in the lactating mammary gland, and subsequent IgA accumulation in milk (Wilson and Butcher, 2004). The cloned human CCR10-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR10 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR10 and its ligands.

    Additional Resource :  HTS014C050615 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR10 cDNA (Accession Number: AF215981) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Homey B., et al. (2000) The orphan chemokine receptor G protein-coupled receptor-2 (GPR-2, CCR10) bind the skin-associated chemokine CCL27 (CTACK/ALP/ILC). J. Immunol. 164: 3465-3470.
    Reference 2: 2. Homey B., et al. (2002) CCL27-CCR10 interactions regulate T cell-mediated skin inflammation. Nat. Med. 8: 157-165.
    Reference 3: 3. Pan J., et al. (2000) A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues. J. Immunol. 165: 2943-2949.
    Reference 4: 4. Wang W., et al. (2000) Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2). J. Biol. Chem. 275: 22313-22323.
    Reference 5: 5. Wilson E. and Butcher E.C. (2004) CCL28 controls immunoglobulin (Ig)A plasma cell accumulation in the lactating mammary gland and IgA antibody transfer to the neonate. J. Exp. Med. 200: 805-809.