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HTS013RTA - Ready-to-Assay™ CCR8 Chemokine Receptor Frozen Cells

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    CCR8 is a GPCR that binds primarily to the chemokine I-309 (CCL1) (Roos et al., 1997).  CCR8 is expressed primarily on Th2 cells although its functional role in T cell recruitment to sites of allergic inflammation is controversial (Chensue et al., 2001; Goya et al., 2003).  CCR8+ T-cells are abundant in skin, and rare or absent in the GI tract and peripheral blood, indicating a role in skin-homing of T-cells (Schaerli et al., 2004).  In addition, monocyte-derived dendritic cells express CCR8 and utilize CCR8 during mobilization from skin to the lymph node  (Qu et al., 2004). Cloned human CCR8-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR8 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR8.

    Additional Resource: HTS013RTA092214_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: U45983
    Protein Targets: CCR8
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: CCR8 cDNA (Accession Number: U45983)
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Chensue SW et al. (2001) Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J. Exp. Med. 193:573-84.
    Reference 2: 2. Goya I et al. (2003) Absence of CCR8 does not impair the response to ovalbumin-induced allergic airway disease. J. Immunol. 170: 2138-46.
    Reference 3: 3. Qu C et al. (2004) Role of CCR8 and other chemokine pathways in the migration of monocyte-derived dendritic cells to lymph nodes. J. Exp. Med. 200: 1231-1241.
    Reference 4: 4. Roos RS et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J. Biol. Chem.. 272: 17251-17254.
    Reference 5: 5. Schaerli P et al. (2004) A skin-selective homing mechanism for human immune surveillance T cells. J. Exp. Med. 199: 1265-75