HTS013M - ChemiSCREEN™ CCR8 Chemokine Receptor Membrane Preparation

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    CCR8 is a GPCR that binds primarily to the chemokine I-309 (CCL1) (Roos et al., 1997). CCR8 is expressed primarily on Th2 cells although its functional role in T cell recruitment to sites of allergic inflammation is controversial (Chensue et al., 2001; Goya et al., 2003). CCR8+ T-cells are abundant in skin and rare or absent in the GI tract and peripheral blood, indicating a role in skin-homing of T-cells. (Schaerli et al., 2004). In addition, monocyte-derived dendritic cells express CCR8 and utilize CCR8 during mobilization from skin to the lymph node (Qu et al., 2004). CCR8 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR8 interactions with I-309.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: Radioligand Binding Assay
    Species: Human CCR8 (Accession number U45983)
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous CCR8 expression.
    Reference 1: 1. Chensue SW et al. (2001) Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J. Exp. Med. 193:573-84.
    Reference 2: 2. Goya I et al. (2003) Absence of CCR8 does not impair the response to ovalbumin-induced allergic airway disease. J. Immunol. 170: 2138-46.
    Reference 3: 3. Qu C et al. (2004) Role of CCR8 and other chemokine pathways in the migration of monocyte-derived dendritic cells to lymph nodes. J. Exp. Med. 200: 1231-1241.
    Reference 4: 4. Roos RS et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J. Biol. Chem. 272: 17251-17254.
    Reference 5: 5. Schaerli P et al. (2004) A skin-selective homing mechanism for human immune surveillance T cells. J. Exp. Med. 199: 1265-75.