HTS013C - ChemiSCREEN™ Human CCR8 Chemokine Receptor Calcium-Optimized Stable Cell Line

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HTS013C
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    CCR8 is a GPCR that binds primarily to the chemokine I-309 (CCL1) (Roos et al., 1997). CCR8 is expressed primarily on Th2 cells although its functional role in T cell recruitment to sites of allergic inflammation is controversial (Chensue et al., 2001; Goya et al., 2003). CCR8+ T-cells are abundant in skin, and rare or absent in the GI tract and peripheral blood, indicating a role in skin-homing of T-cells (Schaerli et al., 2004). In addition, monocyte-derived dendritic cells express CCR8 and utilize CCR8 during mobilization from skin to the lymph node (Qu et al., 2004). The cloned human CCR8-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR8 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium s

    Additional Resource :  HTS013C050615 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR8 cDNA (Accession Number: U45983) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Chensue SW et al. (2001) Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J. Exp. Med. 193:573-84.
    Reference 2: 2. Goya I et al. (2003) Absence of CCR8 does not impair the response to ovalbumin-induced allergic airway disease. J. Immunol. 170: 2138-46.
    Reference 3: 3. Qu C et al. (2004) Role of CCR8 and other chemokine pathways in the migration of monocyte-derived dendritic cells to lymph nodes. J. Exp. Med. 200: 1231-1241.
    Reference 4: 4. Roos RS et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J. Biol. Chem.. 272: 17251-17254.
    Reference 5: 5. Schaerli P et al. (2004) A skin-selective homing mechanism for human immune surveillance T cells. J. Exp. Med. 199: 1265-75.
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