Calcium Flux Assay

HTS012C - ChemiSCREEN™ Human CCR7 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    CCR7 is a GPCR that binds to CC chemokine ligands MIP-3β (ELC/CCL19) and 6Ckine (CCL21) (Yoshida et al., 1997). These ligands are expressed in the secondary lymphoid organs, and binding to CCR7 expressed in naïve T cells, B cells and dendritic cells directs migration of these cells to sites of antigen presentation (Förster et al., 1999). Inhibition of CCR7/ligand interactions inhibits contact sensitivity, delayed type hypersensitivity, and graft vs host disease in experimental models (Förster et al., 1999; Sasaki et al. 2003). In addition, CCR7 expression by breast cancer, melanoma and other malignant cells are associated with lymph node metastasis (Muller et al., 2001; Payne and Cornelius, 2002). The cloned human CCR7-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR7 expression on the cell surface and contains high levels of the promiscuous G protein Gα16 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR7 and its ligands.

    Additional Resource :  HTS012C050615 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR7 cDNA (Accession Number: L08176) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Förster R et al. (1999) CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell 99: 23-33.
    Reference 2: 2. Muller A et al. (2001) Involvement of chemokine receptors in breast cancer metastasis. Nature 410: 50-6.
    Reference 3: 3. Payne AS and Cornelius LA (2002) The role of chemokines in melanoma tumor growth and metastasis. J. Invest. Dermatol. 118: 915-22.
    Reference 4: 4. Sasaki M et al. (2003) Antagonist of secondary lymphoid-tissue chemokine (CCR ligand 21) prevents the development of chronic graft-versus-host disease in mice. J. Immunol. 170: 588-96.
    Reference 5: 5. Yoshida, R, et al. (1997) Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J. Biol. Chem. 272: 13803-9.