HTS011C - ChemiSCREEN™ Human CCR6 Chemokine Receptor Calcium-Optimized Stable Cell Line

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HTS011C
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    CCR6 is a GPCR that binds to a single chemokine, CCL20 (also known as MIP-3α, LARC or Exods-1), as well as several members of the beta-defensin family of antimicrobial peptides (Schutyser, 2003). Immature dendritic cells, B-cells, and effector/memory T-cells express CCR6, and the receptor promotes inflammation during the development of rheumatoid arthritis, inflammatory bowel disease, and graft-versus-host disease (Varona et al., 2003; Kleinewietfeld et al., 2005; Varona et al., 2005). The cloned human CCR6-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR6 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR6 and its ligands.

    Additional Resource :  HTS011C050615 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR6 cDNA (Accession Number: U45984- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Kleinewietfeld M et al. (2005) CCR6 expression defines regulatory effector/memory-like cells within the CD25(+)CD4+ T-cell subset. Blood 105: 2877-86.
    Reference 2: 2. Schutyser E (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14: 409-26.
    Reference 3: 3. Varona R et al. (2003) CCR6 has a non-redundant role in the development of inflammatory bowel disease. Eur. J. Immunol. 33: 2937-46.
    Reference 4: 4. Varona R et al. (2005) CCR6 regulates CD4+ T-cell-mediated acute graft-versus-host disease responses. Blood 106: 18-26.
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