Calcium Flux Assay

HTS009C - ChemiScreen™ Human CCR4 Receptor Calcium-Optimized Stable Cell Line

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HTS009C
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    CCR4 is a GPCR that is activated by chemokines TARC (thymus and activation regulated chemokine) and MDC (macrophage-derived chemokine) (Olsen and Ley, 2001). NK cells, Th2 cells, cutaneous memory T cells, macrophages and platelets express CCR4 and respond to TARC and MDC (Andrew et al., 2001; Inngjerdingen et al., 2000; Gear et al., 2001; Soler et al., 2003). CCR4-null mice display reduced airway hyperresponsiveness to fungal spores, lipopolysaccharide-induced endotoxic shock, and cardiac allograft rejection (Chvatchko et al., 2000; Schuh et al., 2002; Huser et al., 2005). In addition, antibody –mediated antagonism of MDC inhibits development of insulitis and diabetes in NOD mice (Kim et al., 2002). The cloned human CCR4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CCR4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR4 and its ligands.

    Additional Resource :  HTS009C050515 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-5, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR4 cDNA (Accession Number: X85740) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Andrew DP et al., (2001) C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential. J. Immunol. 166: 103-11.
    Reference 2: 2. Chvatchko Y et al. (2000) A key role for CC chemokine receptor 4 in lipopolysaccharide-induced endotoxic shock. J. Exp. Med. 191: 1755-64.
    Reference 3: 3. Gear AR et al. (2001) Adenosine diphosphate strongly potentiates the ability of the chemokines MDC, TARC, and SDF-1 to stimulate platelet function. Blood 97: 937-45
    Reference 4: 4. Huser N et al. (2005) CCR4-deficient mice show prolonged graft survival in a chronic cardiac transplant rejection model. Eur. J. Immunol. 35: 128-138.
    Reference 5: 5. Inngjerdingen M et al. (2000) Human NK cells express CC chemokine receptors 4 and 8 and respond to thymus and activation-regulated chemokine, macrophage-derived chemokine, and I-309. J. Immunol. 164: 4048-54
    Reference 6: 6. Kim SH et al. (2002) CCR4-bearing T cells participate in autoimmune diabetes. J. Clin. Invest. 110: 1675-1686.
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