Calcium Flux Assay

HTS008C - ChemiSCREEN™ Human Recombinant CCR3 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    Eosinophils are major effector cells implicated in a number of chronic inflammatory diseases in humans, particularly bronchial asthma and allergic rhinitis. The chemokine receptor 3 (CCR3), a GPCR activated by chemokines eotaxin 1/2, MCP-3, MCP-4, and RANTES, mediates selective recruitment of eosinophils into tissue and thus has recently become an attractive biological target for therapeutic intervention (Fujisawa et al., 2000). It is widely expressed on cells involved in allergic inflammation, such as basophils, mast cells, airway epithelial cells, and potentially TH2 T-lymphocytes. Allergen-induced eosinophil infiltration into airways is reduced or eliminated in CCR3 and eotaxin 1/2 knockout mice mice and in mice treated with antibodies directed against CCR3 (Grimaldi et al., 1999; Fulkerson et al., 2006). CCR3 antagonists are currently being developed for the treatment of asthma and other allergic disorders. Cloned human CCR3 receptor-expressing ChemiScreen cells were constructed by stable transfection of Chem-1 cells with CCR3 and contain high levels of the promiscuous G protein Ga15 to couple the receptor to the calcium signaling pathway. These stability-tested cells are ready for fluorescence-based assays for agonists, antagonists and modulators at the CCR3 receptor.

    Additional Resource :  HTS008C011615 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: CCR3 cDNA (Accession Number: U28694) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Fujisawa T et al. (2000). Chemokines induce eosinophil degranulation through CCR-3. J. Allergy Clin. Immunol. 106: 507–513.
    Reference 2: 2. Fulkerson PC et al. (2006) A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc. Natl. Acad. Sci. USA 103: 16418-16423.
    Reference 3: 3. Grimaldi JC et al. (1999). Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3). J. Leukoc. Biol. 65: 846–853.