HTS007M - ChemiSCREEN™ CCR2B Chemokine Receptor Membrane Preparation

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    CCR2 is a GPCR that is expressed on monocytes, dendritic cells, natural killer cells, basophils and neutrophils, and binds to MCP-1, 3 and 4, members of the MCP (monocyte chemoattractant protein) family of chemokines (Olson and Ley, 2002). Alternative splicing of the CCR2 gene results in two variants, CCR2A and CCR2B, which differ in their C-terminal tails (Wong and Charo, 1997). CCR2 plays a protective role in some conditions: mice lacking the gene encoding CCR2 develop age-related macular degeneration (Ambati et al., 2003), and in experimental models of rheumatoid arthritis, Ccr2-null mice develop a phenotype of greater severity than wild-type mice (Quinones et al., 2004). However, CCR2 appears to promote the pathogenesis of graft-versus-host disease and pulmonary and kidney fibrosis (Kitagawa et al., 2004; Rao et al., 2003). CCR2B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR2B interactions with MCP-1.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: Radioligand Binding Assay
    Species: Human CCR2B (Accession number U03905)
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous CCR2B expression.
    Reference 1: 1. Ambati J et al. (2003) An animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice. Nat. Med. 9: 1390-1397.
    Reference 2: 2. Kitagawa K et al. (2004) Blockade of CCR2 ameliorates progressive fibrosis in kidney. Am. J. Pathol. 165: 237-246.
    Reference 3: 3. Olson TS and Ley K (2002) Chemokines and chemokine receptors in leukocyte trafficking. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R7-28.
    Reference 4: 4. Quinones MP et al. (2004) Experimental arthritis in CC chemokine receptor 2-null mice closely mimics severe human rheumatoid arthritis. J. Clin. Invest. 113: 856-66.
    Reference 5: 5. Rao AR et al. (2003) CC chemokine receptor 2 expression in donor cells serves an essential role in graft-versus-host-disease. J. Immunol. 171: 4875-4885.
    Reference 6: 6. Wong LM et al. (1997) Organization and differential expression of the human monocyte chemoattractant protein 1 receptor gene. Evidence for the role of the carboxyl-terminal tail in receptor trafficking. J. Biol. Chem. 272: 1038-45.