HTS007C - ChemiSCREEN™ Human CCR2B Receptor Calcium-Optimized Stable Cell Line

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HTS007C
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    CCR2 is a GPCR that is expressed on monocytes, dendritic cells, natural killer cells, basophils and neutrophils, and binds to MCP-1, 3 and 4, members of the MCP (monocyte chemoattractant protein) family of chemokines (Olson and Ley, 2002). Alternative splicing of the CCR2 gene results in two variants, CCR2A and CCR2B, that differ in their C-terminal tails (Wong and Charo, 1997). CCR2 plays a protective role in some conditions: mice lacking the gene encoding CCR2 develop age-related macular degeneration (Ambati et al., 2003), and in experimental models of rheumatoid arthritis, Ccr2-null mice develop a phenotype of greater severity than wild-type mice (Quinones et al., 2004). However, CCR2 appears to promote the pathogenesis of graft-versus-host disease and pulmonary and kidney fibrosis (Kitagawa et al., 2004; Rao et al., 2003). The cloned human CCR2B-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR2B expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of CCR2B.

    Additional Resource :  HTS007C050515 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CCR2B cDNA (Accession Number: U03905) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Ambati J et al. (2003) An animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice. Nat. Med. 9: 1390-1397.
    Reference 2: 2. Kitagawa K et al. (2004) Blockade of CCR2 ameliorates progressive fibrosis in kidney. Am. J. Pathol. 165: 237-246.
    Reference 3: 3. Olson TS and Ley K (2002) Chemokines and chemokine receptors in leukocyte trafficking. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R7-28.
    Reference 4: 4. Quinones MP et al. (2004) Experimental arthritis in CC chemokine receptor 2-null mice closely mimics severe human rheumatoid arthritis. J. Clin. Invest. 113: 856-66.
    Reference 5: 5. Rao AR et al. (2003) CC chemokine receptor 2 expression in donor cells serves an essential role in graft-versus-host-disease. J. Immunol. 171: 4875-4885.
    Reference 6: 6. Wong LM et al. (1997) Organization and differential expression of the human monocyte chemoattractant protein 1 receptor gene. Evidence for the role of the carboxyl-terminal tail in receptor trafficking. J. Biol. Chem. 272: 1038-45.
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