HTS002C - ChemiSCREEN™ Human CXCR2 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    CXCR2 is a 7-TM G-protein coupled receptor that binds to the chemokines GROa, GROb, GROg, IL-8, ENA-78, NAP-2 and GCP-2 (Olson and Ley, 2002). Neutrophils, mast cells and microvascular endothelial cells express CXCR2, and interactions of CXCR2 with its ligands promotes chemotaxis of these cell types (Heidemann et al., 2003; Nilsson et al., 1999; White et al., 1998). Studies with mice lacking CXCR2 indicate that CXCR2 promotes growth of primary tumors and secondary metastases (Keane et al., 2004), and plays an essential role in hyperoxia-induced lung injury (Sue et al., 2004). In addition, cytomegalovirus encodes a CXCR2-binding chemokine, vCXC-1, that promotes neutrophil migration to infected cells (Penfold et al., 1999). The cloned human CXCR2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CXCR2 expression on the cell surface and contains high levels of the promiscuous G protein G?15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CXCR2 and its ligands.

    Additional Resource: HTS002C050615_Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CXCR2 cDNA (Accession Number: M73969) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Heidemann J, et al. (2003) Angiogenic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2. J. Biol. Chem. 278: 8508-15.
    Reference 2: 2. Keane MP, et al. (2004) Depletion of CXCR2 inhibits tumor growth and angiogenesis in a murine model of lung cancer. J. Immunol. 172: 2853-60.
    Reference 3: 3. Nilsson G, et al. (1999) Mast cell migratory response to interleukin-8 is mediated through interaction with chemokine receptor CXCR2/Interleukin-8RB. Blood 93: 2791-7.
    Reference 4: 4. Olson TS and Ley K (2002) Chemokines and chemokine receptors in leukocyte trafficking. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R7-28.
    Reference 5: 5. Penfold ME, et al. (1999) Cytomegalovirus encodes a potent alpha chemokine. Proc Natl Acad Sci USA 96: 9839-44
    Reference 6: 6. Sue RD, et al. (2004) CXCR2 is critical to hyperoxia-induced lung injury. J. Immunol. 172: 3860-8