- Assay Kits
- Proteins & Enzymes
- Membrane Preps
- Frozen Cells
- Cell Lines
To meet the demands of HTS and selectivity profiling, our ChemiScreen™ stable cell lines were designed to provide robust data on a uniform platform. Most of our ChemiScreen™ cell lines were created in our proprietary host background, Chem-1, which contains endogenous expression of Gα15, a promiscuous G protein, to help funnel signaling to a common calcium readout, regardless of G protein coupling status. Additional second messenger signaling, e.g., cAMP and ERK phosphorylation, has been found for many targets, which can be used to perform orthogonal assay confirmation.
Advantages of ChemiScreen™ GPCR cell lines:
ChemiBrite™ GPCR Cell Lines for Luminescent Assays
Engineered to flash with brilliance upon GPCR activation, our ChemiBrite™ stable cell lines were developed to provide the greatest readout flexibility, including luminescent and fluorescent calcium flux as well as cAMP. The ChemiBrite™ cells express a proprietary mutant version of clytin, a calcium activated photoprotein. These cells are available as either "tool box" reagents (parental line) to develop your own GPCR cell lines or as fully tested cell lines stably overexpressing a GPCR of interest.
Advantages of ChemiBrite™ GPCR cell lines:
Review our ChemiScreen™ and ChemiBrite™ cell lines here.
For additional GPCR cell lines, visit Eurofins DiscoverX.