Plasmin Human Chymotrypsin Serine Peptidase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Plasmin, a trypsin-like serine protease, is generated by proteolytic cleavage of plasminogen, a circulating proenzyme in the liver and secreted in plasma. As a fibrinolytic enzyme, plasmin degrades fibrin into soluble fibrin degradation products. Apart from blood clot dissolution, plasmin is involved in processes such as wound healing, angiogenesis, inflammation, tumor progression and neuronal degeneration. Plasmin is also implicated to play a role in the conversion of proenzyme involved in the complement pathways and in the generation of insulin and bradykinin.
Family:
Protease
Sub Family:
Chymotrypsin
Class:
Serine Peptidase
Protein Name:
Plasmin
Uniprot Number:
P00747
Gene Name:
PLG
Gene ID:
5340
Accession Number:
NM_000301
Organism:
Human
Construct Details:
Endogenous
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human plasma plasmin is used. Test compound and/or vehicle is preincubated with 2 mU/ml enzyme in modified Tris-HCl buffer pH 7.6 for 15 minutes at 37˚C. The reaction is initiated by addition of 50 μM D-Val-Leu-Lys-AMC for another 60-minute incubation period. Determination of the amount of AMC formed is read spectrofluorimetrically at Excitation: 360 nm; Emission: 465 nm. Compounds are screened at 10 μM.
Substrate:
50 μM D-Val-Leu-Lys-AMC
Incubation:
60 min at 37°C
Control Inhibitor:
Patamostat
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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