OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
This assay is intended to assess if a test compound is a substrate for the CYP3A5 enzyme. The stability of the test compound is determined by LCMS after incubation of the test compound with recombinant human CYP3A5
Family:
CYP450
Sub Family:
CYP3
Protein Name:
CYP3A5
Uniprot Number:
P20815
Gene Name:
CYP3A5
Gene ID:
1577
Gene Aliases:
PCN3|P450PCN3|CP35
Organism:
Human
Construct Details:
Full length, recombi
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Detection Method:
HPLC-MS/MS
Measured Response:
Peak Area Response
Testing Information
Substrate:
Test compound
Incubation:
15 min pre-incubation and 0, 15, 30, 60, and 90 min with NADPH at 37°C
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
15 Business Days
Clinical Relevance
Adverse / Beneficial Action:
Inhibition may result in altered drug metabolism.
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
RELATED SOLUTIONS
CYP3A5 inhibition (recombinant, BFC substrate) - US
Item: 786
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
