Free Radical Scavenger Synthetic ABTS Radical Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
A free radical is an atom or molecule that has one or more unpaired electrons. The evidence that reactive species of oxygen (O2-, H2O2, OH- and NO2+) play an important role in pulmonary oxygen toxicity, inflammation, neurodegenerative disorders, ischemia/reperfusion injury and cancer, etc. The chromogen 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS+) can be directly generated by the enzymatic system formed by hydrogen peroxide and horseradish peroxidase in ethanolic media. The ABTS+ generated shows excellent stability for at least 10 minutes, which was used for estimating anti-oxidant activity. This screen can provide an independent and simultaneous evaluation of the low molecular weight and protein antioxidants present in biological samples such as serum.
Family:
Free Radical Scavenger
Organism:
Synthetic
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrophotometry
Measured Response:
Absorbance
Testing Information
Procedure Summary:
Test compound and/or vehicle is incubated with ABTS+ solution (ABTS substrate buffer : H2O2 : Peroxidase = 1 : 1 : 1) for 5 minutes at 25⁰C. Scavenger activity is determined spectrophotometrically by measuring the quenching of the chromagoen radical ( ABTS+) . Compounds are screened at 10 μM.
Substrate:
180 μM ABTS+
Incubation:
5 min at 25°C
Control Inhibitor:
TroloxTM C
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
