EG5 Human Kinesin Enzymatic LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Eg5, a member of the kinesin superfamily, plays a key role in mitosis, orchestrates chromosome segregation in dividing cells. It has been shown Eg5 is overexpressed in many proliferative tissues, targeting Eg5 may lead to mitotic arrest and Eg5 inhibitors displayed potent anticancer activity against some of the mutant tumors. Identification of Eg5 inhibitor may be an important for a novel anticancer therapeutic.
Family:
DNA Repair & Mitotic Regulation
Sub Family:
Kinesin
Protein Name:
EG5
Uniprot Number:
P52732
Gene Name:
KIF11
Gene ID:
3832
Gene Aliases:
Eg5|HKSP|TRIP5
Accession Number:
NM_004523
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrophotometric
Testing Information
Substrate:
ATP (50 µM)
Incubation:
10 min at RT
Control Inhibitor:
S-trityl-L-cysteine
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
Diversity LeadHunter Panel - FR
Item: P9
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