CLIK (STK35) Human Other Protein Kinase Enzymatic Radiometric [10 µM ATP] Kinase LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
STK35 (Serine/threonine kinase 35) is a protein kinase that catalyzes the phosphorylation of serine and threonine residues on target proteins, thereby modulating their activity and function. This kinase interacts with nuclear proteins, playing a significant role in chromatin remodeling and transcriptional regulation. STK35 has been implicated in cellular stress responses and DNA damage repair pathways, suggesting its involvement in maintaining genomic stability. Overexpression of STK35 has been observed in colorectal and breast cancers, correlating with poor prognosis, which indicates its potential as a prognostic biomarker. The multifaceted functions of STK35 in cell cycle regulation, apoptosis, and signal transduction pathways make it an attractive therapeutic target for cancer and other diseases characterized by dysregulated cell signaling. However, inhibition of STK35 may be associated with cardiotoxicity, including hypertension, QT prolongation, and arterial thrombosis.
Family:
Kinase
Sub Family:
Other Protein Kinase
Class:
Protein Ser/Thr
Protein Name:
STK35
Uniprot Number:
Q8TDR2
Protein Aliases:
CLP-36-interacting kinase 1?(CLIK-1);PDLIM1-interacting kinase 1;Serine/threonine-protein kinase 35 L1
Gene Name:
STK35
Gene ID:
140901
Gene Aliases:
bA550O8.2;CLIK1
Accession Number:
NM_080836.2
Organism:
Human
Construct Details:
Full length, recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometry
Measured Response:
Scintillation
Testing Information
Procedure Summary:
Human recombinant protein kinase STK35 expressed in SF21 insect cells is used. Test compound and/or vehicle is preincubated with 2.94 μg/ml enzymeǂ in modified MOPS buffer pH 7.2 for 15 minutes at 37oC. The reaction is initiated by addition of 200 nM RS repeat peptide, 10 mM ATP and 0.05 mCi [g32P]ATP for another 30 minute incubation period and terminated by further addition of 3% H3PO4. An aliquot is removed and counted to determine the amount of [32P]RS repeat peptide formed. Compounds are screened at 10 mM.
Substrate:
200 nM RS repeat peptide
ATP Concentration:
10 uM
Incubation:
30 min at 37°C
Control Inhibitor:
Staurosporine
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
10
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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