CDC25A Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Cell Division Cycle 25A (CDC25A) is a member of the CDC25 family of dual specificity protein tyrosine phosphatases that dephosphorylate and activate cyclin-dependent kinases (CDKs). Mammalian cells express at least three dual phosphatase Cdc25 homologues, named A, B, and C. CDC25A is required for progression from G1 to the S phase in human cells and suggests that this enzyme is part of an auto-amplification loop analogous to that described at the G2/M transition. CDC25A phophatase is an oncogene product and its inhibition may be useful in reducing tumor cell proliferation.
Family:
Phosphatase
Sub Family:
Dual Specificity
Class:
Protein Tyrosine
Protein Name:
CDC25A
Uniprot Number:
P30304
Protein Aliases:
Dual specificity phosphatase Cdc25A
Gene Name:
CDC25A
Gene ID:
993
Accession Number:
NM_201567
Organism:
Human
Construct Details:
Full Length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase CDC25A expressed in E. coli is used. Test compound and/or vehicle is preincubated with 38 ng/ml enzyme‡ for 15 minutes at 37⁰C in modified Tris buffer pH 8.5. The reaction is initiated by addition of 15 μM 3-O-methylfluorescein phosphate for 60 minutes. Determination of the amount of 3-O-methylfluorescein formed is read spectrofluorimetrically at 471 nm/530 nm. Compounds are screened at 10 μM.
Substrate:
15 µM 3-O-methylfluorescein phosphate
Incubation:
60 min at 37°C
Control Inhibitor:
NSC 663284
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Adverse / Beneficial Action:
CDC25A phophataseis an oncogene productand its inhibition may be useful in reducing tumor cell proliferation.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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