Biological Information

Background Information:

Cathepsin B, L and H are lysosomal enzymes that belong to the papain superfamily of cysteine proteinases. They are thought to be involved in tumor invasion and metastasis. In addition, high levels of Cathepsin B have been found in the synovial fluid of rheumatoid arthritis patients which may degrade collagen. Inhibition of Cathepsin B may reduce inflammation, tumor growth and angiogenesis.

Family:

Protease

Sub Family:

Papain

Class:

Cysteine Peptidase

Protein Name:

Cathepsin B

Uniprot Number:

P07858

Gene Name:

CTSB

Gene ID:

1508

Accession Number:

NM_147780

Organism:

Human

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Spectrophotometric

Testing Information

Substrate:

Z-Arg-Arg-pNa (0.3 mM)

Incubation:

180 min at 37°C

Control Inhibitor:

Leupeptin

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

16760358

Name

cathepsin inhibitor III

Measurement

4.54nM - IC50

PubChem ID

123985

Name

E-64

Measurement

2.65nM - IC50

PubChem ID

443119

Name

chymostatin

Measurement

1700nM - IC50

PubChem ID

13209094

Name

Leupeptin

Measurement

32.4nM - IC50

Clinical Relevance

Therapeutic Area:

Inflammation/Allergy

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

RELATED SOLUTIONS

Item: P411