OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Aurora family of kinases, contained at least three proteins (Aurora A, B and C), phosphorylate histone H3 and provide a direct path to the nucleosome. Aurora-B (AURAKB, AIK2, AIM1, ARK2, STK12) is needed for metaphase alignment of chromosomes, sister chromatid separation and cytokinesis. Overexpression of Aurora-B is found in several types of cancer and causes dramatic increases in genomic instability.
Family:
Kinase
Sub Family:
Other Protein Kinase
Class:
Protein Ser/Thr
Protein Name:
Aurora-B
Uniprot Number:
Q96GD4
Protein Aliases:
aurora-B|aurora-1|protein phosphatase 1, regulatory subunit 48
Gene Name:
AURKB
Gene ID:
9212
Gene Aliases:
Aik2|IPL1|AurB|AIM-1|ARK2|STK5|PPP1R48
Accession Number:
NM_004217
Organism:
Human
Construct Details:
Full length recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Procedure Summary:
Aurora-B (h) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 30 µM AKRRRLSSLRA, 10 mM MgAcetate and [g- 33P]-ATP (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid to a 9 concentration of 0.5%. An aliquot of the reaction is then spotted onto a filter and washed four times for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting.
Substrate:
30 uM AKRRRLSSLRA
Tracer:
33P
ATP Concentration:
[ATP]= 10µM (Km= 9µM )
Incubation:
40 min at Room temperature
Control Inhibitor:
Staurosporine
Test Sample Requirements:
Please provide compounds dissolved in 100% DMSO (PBS or HBSS prohibited).
Stock solution should be ≥50× the final assay concentration (a 10 mM stock is also acceptable).
Required volume:
– <60 kinase assays: 120 µL of 50× stock
– ≥60 assays: 420 µL of 50× stock
If you supply 10 mM stocks or powder (pre-weighed), we will apply the appropriate conversion.
For batches of ~50 compounds supplied as solids, the TAT will be extended.
Minimum Order Quantity:
2
Turnaround Time
Standard:
6 Business Days
Service Scheduling Note:
Assay is run on a weekly schedule beginning on Friday. Compounds and purchase order must be received on or before 13:00 CET Thursday for the order to be included on the service run that begins on Friday of the same week.
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Adverse / Beneficial Action:
Inhibition of Aurora-B may be useful in the treatment of several solid tumors including breast, ovary, prostate, pancreas and colorectal cancer.
Additional Information
Brand:
LeadHunter
Testing Location:
KP - France - Celle L'Evescault
RELATED SOLUTIONS
Aurora-B (AURKB) Human Other Protein Kinase Enzymatic Radiometric [10 uM ATP] KinaseProfiler LeadHunter Assay - FR
Item: 14-835KP10
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