Biological Information

Background Information:

Angiotensin-converting enzyme 2 (ACE2), identified human homolog of ACE, is a novel metalloprotease with specificity, tissue distribution (heart, lungs and kidneys), and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal function. Inhibitors of ACE2-specific peptide may be useful in the treatment of hypertension and other cardiovascular diseases.

Family:

Protease

Sub Family:

Angiotensin-Converting

Class:

Metallo Peptidase

Protein Name:

ACE2

Uniprot Number:

Q9BYF1

Protein Aliases:

peptidyl-dipeptidase A

Gene Name:

ACE2

Gene ID:

59272

Accession Number:

XM_011545551

Organism:

Human

Construct Details:

Recombinant

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Fluorimetry

Measured Response:

Fluorescence

Testing Information

Substrate:

Mca-Tyr-Val-Ala-Asp-Pro-Ala-Lys-(DNP)-OH (10 µM)

Incubation:

20 min at RT

Control Inhibitor:

Ac-GG-26-NH2

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

6049

Name

EDTA

Measurement

400nM - IC50

PubChem ID

44093

Name

Captopril

Measurement

- IC50

PubChem ID

Name

Ac-GG-26-NH2

Measurement

400nM - IC50

PubChem ID

Name

chloro-2-benzene

Measurement

2000nM - IC50

Clinical Relevance

Therapeutic Area:

Cardiovascular

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

RELATED SOLUTIONS

Item: P22-p