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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
GSK-690693
Highlight These Spots (
fill
):
GSK-690693|AKT1|2.2
GSK-690693|AKT2|2.1
GSK-690693|AKT3|3
GSK-690693|ARK5|420
GSK-690693|CAMK1D|2500
GSK-690693|CAMK4|370
GSK-690693|CHEK2|5300
GSK-690693|CIT|200
GSK-690693|CLK2|730
GSK-690693|DAPK1|3000
GSK-690693|DAPK3|560
GSK-690693|DCAMKL1|2400
GSK-690693|DCAMKL3|110
GSK-690693|GRK1|610
GSK-690693|GRK7|380
GSK-690693|GSK3A|5200
GSK-690693|GSK3B|100
GSK-690693|HIPK1|510
GSK-690693|HIPK2|390
GSK-690693|HIPK3|200
GSK-690693|LATS2|140
GSK-690693|MAP3K2|340
GSK-690693|MAP3K3|880
GSK-690693|MARK2|720
GSK-690693|MELK|890
GSK-690693|MST4|5400
GSK-690693|NLK|3400
GSK-690693|PAK1|550
GSK-690693|PAK2|270
GSK-690693|PAK4|18
GSK-690693|PAK6|21
GSK-690693|PAK7|8.9
GSK-690693|PDGFRB|3900
GSK-690693|PIM1|940
GSK-690693|PIM2|680
GSK-690693|PIM3|750
GSK-690693|PIP5K1C|1500
GSK-690693|PKAC-alpha|19
GSK-690693|PKAC-beta|13
GSK-690693|PKN1|34
GSK-690693|PKN2|3100
GSK-690693|PRKCD|54
GSK-690693|PRKCE|5.3
GSK-690693|PRKCH|2.4
GSK-690693|PRKCI|250
GSK-690693|PRKCQ|16
GSK-690693|PRKD1|3500
GSK-690693|PRKD3|990
GSK-690693|PRKG1|210
GSK-690693|PRKG2|3.1
GSK-690693|PRKR|2000
GSK-690693|PRKX|7.2
GSK-690693|ROCK1|280
GSK-690693|ROCK2|200
GSK-690693|RPS6KA4(Kin.Dom.2-C-terminal)|51
GSK-690693|RPS6KA5(Kin.Dom.2-C-terminal)|160
GSK-690693|SGK3|1900
GSK-690693|SIK2|6900
GSK-690693|SNARK|86
GSK-690693|STK36|2600
GSK-690693|TAOK1|620
GSK-690693|TAOK2|1400
GSK-690693|TAOK3|9300
GSK-690693|ULK1|5900
GSK-690693|ULK2|2300
GSK-690693|YSK4|33
GSK-690693|AKT1
GSK-690693|AKT2
GSK-690693|AKT3
GSK-690693|ARK5
GSK-690693|CAMK1D
GSK-690693|CAMK4
GSK-690693|CHEK2
GSK-690693|CIT
GSK-690693|CLK2
GSK-690693|DAPK1
GSK-690693|DAPK3
GSK-690693|DCAMKL1
GSK-690693|DCAMKL3
GSK-690693|GRK1
GSK-690693|GRK7
GSK-690693|GSK3A
GSK-690693|GSK3B
GSK-690693|HIPK1
GSK-690693|HIPK2
GSK-690693|HIPK3
GSK-690693|LATS2
GSK-690693|MAP3K2
GSK-690693|MAP3K3
GSK-690693|MARK2
GSK-690693|MELK
GSK-690693|MST4
GSK-690693|NLK
GSK-690693|PAK1
GSK-690693|PAK2
GSK-690693|PAK4
GSK-690693|PAK6
GSK-690693|PAK7
GSK-690693|PDGFRB
GSK-690693|PIM1
GSK-690693|PIM2
GSK-690693|PIM3
GSK-690693|PIP5K1C
GSK-690693|PKAC-alpha
GSK-690693|PKAC-beta
GSK-690693|PKN1
GSK-690693|PKN2
GSK-690693|PRKCD
GSK-690693|PRKCE
GSK-690693|PRKCH
GSK-690693|PRKCI
GSK-690693|PRKCQ
GSK-690693|PRKD1
GSK-690693|PRKD3
GSK-690693|PRKG1
GSK-690693|PRKG2
GSK-690693|PRKR
GSK-690693|PRKX
GSK-690693|ROCK1
GSK-690693|ROCK2
GSK-690693|RPS6KA4(Kin.Dom.2-C-terminal)
GSK-690693|RPS6KA5(Kin.Dom.2-C-terminal)
GSK-690693|SGK3
GSK-690693|SIK2
GSK-690693|SNARK
GSK-690693|STK36
GSK-690693|TAOK1
GSK-690693|TAOK2
GSK-690693|TAOK3
GSK-690693|ULK1
GSK-690693|ULK2
GSK-690693|YSK4
GSK-690693|AKT1|2.2
GSK-690693|AKT2|2.1
GSK-690693|AKT3|3
GSK-690693|ARK5|420
GSK-690693|CAMK1D|2500
GSK-690693|CAMK4|370
GSK-690693|CHEK2|5300
GSK-690693|CIT|200
GSK-690693|CLK2|730
GSK-690693|DAPK1|3000
GSK-690693|DAPK3|560
GSK-690693|DCAMKL1|2400
GSK-690693|DCAMKL3|110
GSK-690693|GRK1|610
GSK-690693|GRK7|380
GSK-690693|GSK3A|5200
GSK-690693|GSK3B|100
GSK-690693|HIPK1|510
GSK-690693|HIPK2|390
GSK-690693|HIPK3|200
GSK-690693|LATS2|140
GSK-690693|MAP3K2|340
GSK-690693|MAP3K3|880
GSK-690693|MARK2|720
GSK-690693|MELK|890
GSK-690693|MST4|5400
GSK-690693|NLK|3400
GSK-690693|PAK1|550
GSK-690693|PAK2|270
GSK-690693|PAK4|18
GSK-690693|PAK6|21
GSK-690693|PAK7|8.9
GSK-690693|PDGFRB|3900
GSK-690693|PIM1|940
GSK-690693|PIM2|680
GSK-690693|PIM3|750
GSK-690693|PIP5K1C|1500
GSK-690693|PKAC-alpha|19
GSK-690693|PKAC-beta|13
GSK-690693|PKN1|34
GSK-690693|PKN2|3100
GSK-690693|PRKCD|54
GSK-690693|PRKCE|5.3
GSK-690693|PRKCH|2.4
GSK-690693|PRKCI|250
GSK-690693|PRKCQ|16
GSK-690693|PRKD1|3500
GSK-690693|PRKD3|990
GSK-690693|PRKG1|210
GSK-690693|PRKG2|3.1
GSK-690693|PRKR|2000
GSK-690693|PRKX|7.2
GSK-690693|ROCK1|280
GSK-690693|ROCK2|200
GSK-690693|RPS6KA4(Kin.Dom.2-C-terminal)|51
GSK-690693|RPS6KA5(Kin.Dom.2-C-terminal)|160
GSK-690693|SGK3|1900
GSK-690693|SIK2|6900
GSK-690693|SNARK|86
GSK-690693|STK36|2600
GSK-690693|TAOK1|620
GSK-690693|TAOK2|1400
GSK-690693|TAOK3|9300
GSK-690693|ULK1|5900
GSK-690693|ULK2|2300
GSK-690693|YSK4|33
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
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Spot options:
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Outline?
Outline thickness:
1
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3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
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Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
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Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
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Spot options:
Spot Color transparency level:
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Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
GSK-690693
size/quality
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White Space Border:
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Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
GSK-690693
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* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Sites\eCOMServices\US\PROD\Applications\FrontOffice\StudyManagement\TreeSpot\HTSTools\TreeImages\180_yqjudetoi2mzunwwwk4ruc0w\
Open/Browse Image Folder
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GSK-690693
Legend
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Images per Row:
1
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Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
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Locate an Assay
AKT1
AKT2
AKT3
ARK5
CAMK1D
CAMK4
CIT
CLK2
DAPK3
DCAMKL1
DCAMKL3
GRK1
GRK7
GSK3B
HIPK1
HIPK2
HIPK3
LATS2
MAP3K2
MAP3K3
MARK2
MELK
PAK1
PAK2
PAK4
PAK6
PAK7
PIM1
PIM2
PIM3
PIP5K1C
PKAC-alpha
PKAC-beta
PKN1
PRKCD
PRKCE
PRKCH
PRKCI
PRKCQ
PRKD3
PRKG1
PRKG2
PRKR
PRKX
ROCK1
ROCK2
RPS6KA4(Kin.Dom.2-C-terminal)
RPS6KA5(Kin.Dom.2-C-terminal)
SGK3
SNARK
STK36
TAOK1
TAOK2
ULK2
YSK4
Legend
Data Table View:
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Only Selected Compounds/Trees
TREE
spot
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