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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
PI-103
Highlight These Spots (
fill
):
PI-103|BRAF|2900
PI-103|CDK7|7200
PI-103|CLK1|3900
PI-103|CLK2|5500
PI-103|CLK3|5900
PI-103|CSNK2A1|5400
PI-103|CSNK2A2|2900
PI-103|DAPK1|2400
PI-103|DAPK2|2700
PI-103|DAPK3|840
PI-103|DYRK1B|5700
PI-103|DYRK2|4100
PI-103|FLT3|2500
PI-103|HIPK1|3300
PI-103|HIPK2|290
PI-103|HIPK3|310
PI-103|IKK-alpha|1700
PI-103|JAK1(JH2domain-pseudokinase)|2000
PI-103|LIMK2|3500
PI-103|MRCKA|1800
PI-103|MTOR|12
PI-103|MYLK|1500
PI-103|PIK3C2B|10
PI-103|PIK3C2G|41
PI-103|PIK3CA|1.5
PI-103|PIK3CB|1.7
PI-103|PIK3CD|17
PI-103|PIK3CG|16
PI-103|PIK4CB|3500
PI-103|PIP5K2C|620
PI-103|RAF1|3700
PI-103|RIOK2|770
PI-103|TYK2(JH2domain-pseudokinase)|5900
PI-103|YSK4|930
PI-103|BRAF
PI-103|CDK7
PI-103|CLK1
PI-103|CLK2
PI-103|CLK3
PI-103|CSNK2A1
PI-103|CSNK2A2
PI-103|DAPK1
PI-103|DAPK2
PI-103|DAPK3
PI-103|DYRK1B
PI-103|DYRK2
PI-103|FLT3
PI-103|HIPK1
PI-103|HIPK2
PI-103|HIPK3
PI-103|IKK-alpha
PI-103|JAK1(JH2domain-pseudokinase)
PI-103|LIMK2
PI-103|MRCKA
PI-103|MTOR
PI-103|MYLK
PI-103|PIK3C2B
PI-103|PIK3C2G
PI-103|PIK3CA
PI-103|PIK3CB
PI-103|PIK3CD
PI-103|PIK3CG
PI-103|PIK4CB
PI-103|PIP5K2C
PI-103|RAF1
PI-103|RIOK2
PI-103|TYK2(JH2domain-pseudokinase)
PI-103|YSK4
PI-103|BRAF|2900
PI-103|CDK7|7200
PI-103|CLK1|3900
PI-103|CLK2|5500
PI-103|CLK3|5900
PI-103|CSNK2A1|5400
PI-103|CSNK2A2|2900
PI-103|DAPK1|2400
PI-103|DAPK2|2700
PI-103|DAPK3|840
PI-103|DYRK1B|5700
PI-103|DYRK2|4100
PI-103|FLT3|2500
PI-103|HIPK1|3300
PI-103|HIPK2|290
PI-103|HIPK3|310
PI-103|IKK-alpha|1700
PI-103|JAK1(JH2domain-pseudokinase)|2000
PI-103|LIMK2|3500
PI-103|MRCKA|1800
PI-103|MTOR|12
PI-103|MYLK|1500
PI-103|PIK3C2B|10
PI-103|PIK3C2G|41
PI-103|PIK3CA|1.5
PI-103|PIK3CB|1.7
PI-103|PIK3CD|17
PI-103|PIK3CG|16
PI-103|PIK4CB|3500
PI-103|PIP5K2C|620
PI-103|RAF1|3700
PI-103|RIOK2|770
PI-103|TYK2(JH2domain-pseudokinase)|5900
PI-103|YSK4|930
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
Restore default options
Spot options:
IndianRed
PaleGreen
Gold
PowderBlue
Fill?
Outline?
Outline thickness:
1
2
3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
Right align Text
Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
Show Assay Panel information?
Show Panel Spots?
Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
Dark Tree Branch image?
Hide Lipid, Mutant, ATypical and Pathogen?
Spot options:
Spot Color transparency level:
none
low
medium-low
medium
high
no color
Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
PI-103
size/quality
100
90
80
70
60
50
40
30
% of original
White Space Border:
Yes
No
Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
PI-103
Highlight Panel?
* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Eurofins\PROD\Applications\FrontOffice_Discovery\StudyManagement\TreeSpot\HTSTools\TreeImages\291_wlaowh4sigzq2z4lvwdvqi4v\
Open/Browse Image Folder
View Options:
(These options do not modify the images that have been created, only the view on this page)
PI-103
Legend
Show Selected
Size:
Select Size
small
medium
full size
Images per Row:
1
Legend Resize?
Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
Zoom/Download
Highlight
Locate an Assay
BRAF
BRAF
CSNK2A2
DAPK1
DAPK2
DAPK3
FLT3
FLT3
HIPK2
HIPK3
IKK-alpha
JAK1(JH2domain-pseudokinase)
MRCKA
MTOR
MYLK
PIK3C2B
PIK3C2G
PIK3CA
PIK3CA
PIK3CB
PIK3CD
PIK3CG
PIP5K2C
RIOK2
YSK4
Legend
Data Table View:
Show All Data
Only Selected Compounds/Trees
TREE
spot
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