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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
AT-7519
Highlight These Spots (
fill
):
AT-7519|CAMKK2|2400
AT-7519|CASK|24
AT-7519|CDC2L1|8.4
AT-7519|CDC2L2|5.2
AT-7519|CDC2L5|3.2
AT-7519|CDK11|3300
AT-7519|CDK2|30
AT-7519|CDK3|60
AT-7519|CDK4-cyclinD1|20
AT-7519|CDK5|700
AT-7519|CDK7|2.8
AT-7519|CDK9|5.8
AT-7519|CDKL2|1000
AT-7519|CDKL5|2.6
AT-7519|CLK1|990
AT-7519|CLK2|1700
AT-7519|CLK3|370
AT-7519|CLK4|7000
AT-7519|DCAMKL2|2600
AT-7519|DCAMKL3|1100
AT-7519|DMPK|1100
AT-7519|DYRK1A|120
AT-7519|DYRK1B|290
AT-7519|DYRK2|5400
AT-7519|ERK5|42
AT-7519|ERK8|120
AT-7519|GSK3A|23
AT-7519|GSK3B|11
AT-7519|ICK|8.3
AT-7519|MAK|97
AT-7519|PCTK1|1.1
AT-7519|PCTK2|0.95
AT-7519|PCTK3|14
AT-7519|PFTAIRE2|62
AT-7519|PFTK1|16
AT-7519|SLK|5900
AT-7519|TAOK1|130
AT-7519|TAOK2|50
AT-7519|TAOK3|57
AT-7519|TLK1|2400
AT-7519|CAMKK2
AT-7519|CASK
AT-7519|CDC2L1
AT-7519|CDC2L2
AT-7519|CDC2L5
AT-7519|CDK11
AT-7519|CDK2
AT-7519|CDK3
AT-7519|CDK4-cyclinD1
AT-7519|CDK5
AT-7519|CDK7
AT-7519|CDK9
AT-7519|CDKL2
AT-7519|CDKL5
AT-7519|CLK1
AT-7519|CLK2
AT-7519|CLK3
AT-7519|CLK4
AT-7519|DCAMKL2
AT-7519|DCAMKL3
AT-7519|DMPK
AT-7519|DYRK1A
AT-7519|DYRK1B
AT-7519|DYRK2
AT-7519|ERK5
AT-7519|ERK8
AT-7519|GSK3A
AT-7519|GSK3B
AT-7519|ICK
AT-7519|MAK
AT-7519|PCTK1
AT-7519|PCTK2
AT-7519|PCTK3
AT-7519|PFTAIRE2
AT-7519|PFTK1
AT-7519|SLK
AT-7519|TAOK1
AT-7519|TAOK2
AT-7519|TAOK3
AT-7519|TLK1
AT-7519|CAMKK2|2400
AT-7519|CASK|24
AT-7519|CDC2L1|8.4
AT-7519|CDC2L2|5.2
AT-7519|CDC2L5|3.2
AT-7519|CDK11|3300
AT-7519|CDK2|30
AT-7519|CDK3|60
AT-7519|CDK4-cyclinD1|20
AT-7519|CDK5|700
AT-7519|CDK7|2.8
AT-7519|CDK9|5.8
AT-7519|CDKL2|1000
AT-7519|CDKL5|2.6
AT-7519|CLK1|990
AT-7519|CLK2|1700
AT-7519|CLK3|370
AT-7519|CLK4|7000
AT-7519|DCAMKL2|2600
AT-7519|DCAMKL3|1100
AT-7519|DMPK|1100
AT-7519|DYRK1A|120
AT-7519|DYRK1B|290
AT-7519|DYRK2|5400
AT-7519|ERK5|42
AT-7519|ERK8|120
AT-7519|GSK3A|23
AT-7519|GSK3B|11
AT-7519|ICK|8.3
AT-7519|MAK|97
AT-7519|PCTK1|1.1
AT-7519|PCTK2|0.95
AT-7519|PCTK3|14
AT-7519|PFTAIRE2|62
AT-7519|PFTK1|16
AT-7519|SLK|5900
AT-7519|TAOK1|130
AT-7519|TAOK2|50
AT-7519|TAOK3|57
AT-7519|TLK1|2400
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
Restore default options
Spot options:
IndianRed
PaleGreen
Gold
PowderBlue
Fill?
Outline?
Outline thickness:
1
2
3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
Right align Text
Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
Show Assay Panel information?
Show Panel Spots?
Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
Dark Tree Branch image?
Hide Lipid, Mutant, ATypical and Pathogen?
Spot options:
Spot Color transparency level:
none
low
medium-low
medium
high
no color
Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
AT-7519
size/quality
100
90
80
70
60
50
40
30
% of original
White Space Border:
Yes
No
Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
AT-7519
Highlight Panel?
* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Eurofins\PROD\Applications\FrontOffice_Discovery\StudyManagement\TreeSpot\HTSTools\TreeImages\623_kuc5mf21v12z1ve5igwkqdq0\
Open/Browse Image Folder
View Options:
(These options do not modify the images that have been created, only the view on this page)
AT-7519
Legend
Show Selected
Size:
Select Size
small
medium
full size
Images per Row:
1
Legend Resize?
Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
Zoom/Download
Highlight
Locate an Assay
CAMKK2
CASK
CDC2L1
CDC2L2
CDC2L5
CDK2
CDK3
CDK4-cyclinD1
CDK5
CDK7
CDK9
CDKL2
CDKL5
CLK1
CLK2
CLK3
DCAMKL2
DCAMKL3
DMPK
DYRK1A
DYRK1B
ERK5
ERK8
GSK3A
GSK3B
ICK
MAK
PCTK1
PCTK2
PCTK3
PFTAIRE2
PFTK1
TAOK1
TAOK2
TAOK3
TLK1
Legend
Data Table View:
Show All Data
Only Selected Compounds/Trees
TREE
spot
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