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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
BMS-345541
Highlight These Spots (
fill
):
BMS-345541|CDC2L1|420
BMS-345541|CDC2L2|390
BMS-345541|CDC2L5|800
BMS-345541|CDK7|680
BMS-345541|CDK9|1900
BMS-345541|DCAMKL3|1700
BMS-345541|DYRK2|2100
BMS-345541|ERK3|4300
BMS-345541|ERK5|620
BMS-345541|ERK8|4500
BMS-345541|ERN1|1000
BMS-345541|HIPK4|8100
BMS-345541|IKK-alpha|1700
BMS-345541|IKK-beta|130
BMS-345541|MYLK4|700
BMS-345541|PCTK1|890
BMS-345541|PRKD1|4600
BMS-345541|RPS6KA4(Kin.Dom.2-C-terminal)|2300
BMS-345541|RSK1(Kin.Dom.2-C-terminal)|3300
BMS-345541|RSK4(Kin.Dom.2-C-terminal)|6700
BMS-345541|STK36|6500
BMS-345541|TYK2(JH2domain-pseudokinase)|2000
BMS-345541|YSK4|260
BMS-345541|CDC2L1
BMS-345541|CDC2L2
BMS-345541|CDC2L5
BMS-345541|CDK7
BMS-345541|CDK9
BMS-345541|DCAMKL3
BMS-345541|DYRK2
BMS-345541|ERK3
BMS-345541|ERK5
BMS-345541|ERK8
BMS-345541|ERN1
BMS-345541|HIPK4
BMS-345541|IKK-alpha
BMS-345541|IKK-beta
BMS-345541|MYLK4
BMS-345541|PCTK1
BMS-345541|PRKD1
BMS-345541|RPS6KA4(Kin.Dom.2-C-terminal)
BMS-345541|RSK1(Kin.Dom.2-C-terminal)
BMS-345541|RSK4(Kin.Dom.2-C-terminal)
BMS-345541|STK36
BMS-345541|TYK2(JH2domain-pseudokinase)
BMS-345541|YSK4
BMS-345541|CDC2L1|420
BMS-345541|CDC2L2|390
BMS-345541|CDC2L5|800
BMS-345541|CDK7|680
BMS-345541|CDK9|1900
BMS-345541|DCAMKL3|1700
BMS-345541|DYRK2|2100
BMS-345541|ERK3|4300
BMS-345541|ERK5|620
BMS-345541|ERK8|4500
BMS-345541|ERN1|1000
BMS-345541|HIPK4|8100
BMS-345541|IKK-alpha|1700
BMS-345541|IKK-beta|130
BMS-345541|MYLK4|700
BMS-345541|PCTK1|890
BMS-345541|PRKD1|4600
BMS-345541|RPS6KA4(Kin.Dom.2-C-terminal)|2300
BMS-345541|RSK1(Kin.Dom.2-C-terminal)|3300
BMS-345541|RSK4(Kin.Dom.2-C-terminal)|6700
BMS-345541|STK36|6500
BMS-345541|TYK2(JH2domain-pseudokinase)|2000
BMS-345541|YSK4|260
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
Restore default options
Spot options:
IndianRed
PaleGreen
Gold
PowderBlue
Fill?
Outline?
Outline thickness:
1
2
3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
Right align Text
Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
Show Assay Panel information?
Show Panel Spots?
Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
Dark Tree Branch image?
Hide Lipid, Mutant, ATypical and Pathogen?
Spot options:
Spot Color transparency level:
none
low
medium-low
medium
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no color
Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
BMS-345541
size/quality
100
90
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70
60
50
40
30
% of original
White Space Border:
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Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
BMS-345541
Highlight Panel?
* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Eurofins\PROD\Applications\FrontOffice_Discovery\StudyManagement\TreeSpot\HTSTools\TreeImages\340_a2zqmkghib005pmrbiv41laj\
Open/Browse Image Folder
View Options:
(These options do not modify the images that have been created, only the view on this page)
BMS-345541
Legend
Show Selected
Size:
Select Size
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Images per Row:
1
Legend Resize?
Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
Zoom/Download
Highlight
Locate an Assay
CDC2L1
CDC2L2
CDC2L5
CDK7
CDK9
DCAMKL3
DYRK2
ERK5
ERN1
IKK-alpha
IKK-beta
MYLK4
PCTK1
RPS6KA4(Kin.Dom.2-C-terminal)
TYK2(JH2domain-pseudokinase)
YSK4
Legend
Data Table View:
Show All Data
Only Selected Compounds/Trees
TREE
spot
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