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Eurofins' ChemiBrite cells express a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other photoproteins targeted to the mitochondria. Luminescent calcium assays offer several advantages over fluorescent calcium assays including increased sensitivity and lack of interference from fluorescent compounds.
U20S Cells are derived from a Human Bone Osteosarcoma. Investigators favor U20S Cells for recombinant expression of GPCR and Ion Channel targets due to high transfection efficiency paired with quiescent endogenous expression of many GPCR and Ion Channel targets. The’s cloned U2OS Parental ChemiBrite cells are made by stable transfection of U20S Cells with ChemiBrite clytin. This stability-tested cell line is ideal for recombinant expression of target protein for use in calcium flux assays, for analysis of agonist, antagonist and modulator activity at the target protein.
|Item Unit of Measure:||PK|
|Contents:||2 vials of mycoplasma-free cells, 1 ml per vial.|
|Storage:||Vials are to be stored in liquid N2.|
|Applications:||Calcium Flux Assay, cAMP Accumulation|
|GMO:||This product contains genetically modified organisms.|