Calcium Flux Assay

HTS233LRTA - Ready-to-Assay™ LH Glycoprotein Hormone Receptor Frozen Cells

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  • Product Description

    Luteinizing Hormone (LH), also known as lutropin, is produced by the pituitary gland and is critical for fertility and reproduction. In women, LH modulates ovulation. In men, LH stimulates testosterone production. Several naturally occurring mutations in the LH receptor gene have been associated with human reproductive disorders (Themmen et al.). Together with TSH, hCG and FSH, LH is a member of the glycoprotein hormone family. Each member shares a common α subunit, whereas the β subunit confers functional specificity. Upon activation, LH signals primarily through the Gαqs/adenylyl cyclase/cAMP/PKA pathway as has been shown in native granulosa cells. Cloned human LH receptor-expressing ChemiBrite cells were made by recombinant transfection of HEK293 cells with ChemiBrite clytin, the LH receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway.  The cells have been cryopreserved at an optimal time post-transfection.  Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the LH receptor.

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay, cAMP Accumulation
    Entrez Gene Number: BC156303
    Protein Targets: LH
    Target Sub-family: Glycoprotein
    Host Cell: HEK293
    Exongenous Gene Exoression: Human LHCGR cDNA (Accession Number: BC156303; see CODING SEQUENCE below) and a proprietary mutant clytin photoprotein and promiscuous G protein expressed in a bicistronic vector.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Koppen et al. (2008) A signaling-selective, nanomolar potent allosteric low molecular weight agonist for the human luteinizing hormone receptor. Arch. Pharm.(378)5: 503-514
    Reference 2: 2. Jia X. et al. (1991) Expression of Human Luteinizing Hormone Receptor: Interaction with LH and Chorionic Gonadotropin from Human but not Equine, Rat and Ovine Species. Mol. Endocrinology 5 (6)
    Reference 3: 3. Themmen A. et al. (2000) Mutations of Gonadotropins and Gonadotropin Receptors: Elucidating the Physiology and Pathophysiology of Pituitary-Gonadal Function. Endocrine Review (21)5: 551-583