Calcium Flux Assay

HTS225L - ChemiBrite™ GPR120 Receptor Stable Cell Line

×
×
Price:
$10,295.00
SKU:
HTS225L
Quantity:
Qty:
Description
Other Details
Reviews
Related Products
Product Videos
Warranty
Also Viewed
By Category
  • Product Description

    Oh et al (2010) demonstrated that Omega-3 fatty acid administration to obese mice inhibited inflammation and enhanced insulin sensitivity, but these effects were absent in GPR120 knockout mice. GPR120 agonists may prove to be useful in suppression of chronic inflammation seen in obesity, which could reduce insulin resistance and help restore glucose control. A recent review suggests that the effects of marine n-3 fatty acids on inflammatory markers studied in healthy subjects, those at high risk for developing Cardiovascular Disease, and those with diagnosed Cardiovascular Disease are as yet not conclusive (Myhrstad et al. 2011). Eurofins' cloned human GPR120 receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin and the GPR120 receptor. These stability-tested cells are ready for luminescent analysis of agonists, antagonists and modulators at the GPR120 receptor.

  • Find Similar Products by Category

  • Other Details

    Item Unit of Measure: PK
    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: BC101175.2
    Host Cell: Chem-5, an adherent rat hematopoietic cell line expressing endogenous G·15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exogenous Gene Expression: Human GPR120 cDNA (Accession Number: BC101175.2; see CODING SEQUENCE below) and a proprietary mutant clytin photoprotein expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Oh et al. (2010) GPR120 is an omega-3 fatty acid receptor mediating potent anti-inflammatory and insulinsensitizing effects. Cell. 142(5):687-698.
    Reference 2: 2. Myhrstad et al. (2011) Effect of marine n-3 fatty acids on circulating inflammatory markers in healthy subjects and subjects with cardiovascular risk factors. Inflamm Res. 60(4):309-319.
    Reference 3: 3. Hirasawa et al. (2005). Free fatty acids regulate gut incretion in glucagon-like peptide-1 secretion through GPR120. Nature Medicine:11:90-94
    Reference 4: 4. Briscoe et al. (2006 ) Pharmacological regulation of insulin secretion in MIN6 cells through the fatty acid receptor GPR40: identification of the agonist and antagonist small molecules Br J Pharm 146:619-628
×
×