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HTS185L - ChemiBrite™ EP2 Prostanoid Receptor Stable Cell Line

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    Prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostanoid PGE2 causes pain, vasodilation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. EP2 couples primarily to Gs to increase intracellular cAMP levels. Mice deficient in EP2 receptor showed impaired ovulation and fertilization, salt-sensitive hypertension (Kennedy et al., 1999). It has been shown that EP2 receptors are also involved in cancer associated immunodeficiency. Thus, genetic knockout of the EP2 receptor reduced tumor growth and prolonged survival in mice that had undergone isograft injection of MC26 or Lewis lung carcinoma cells (Yang et al., 2003). Eurofins' cloned human EP2 receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin, the EP2 receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. These stability tested cells are ready for luminescent analysis of agonists, antagonists and modulators at the EP2 receptor.

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    Item Unit of Measure: PK
    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay, cAMP Accumulation
    Entrez Gene Number: NM_000956
    Host Cell: HEK293
    Exogenous Gene Expression: Human PTGER2 cDNA (Accession Number: NM_000956; see CODING SEQUENCE below) and a proprietary mutant clytin photoprotein and promiscuous G protein expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Kennedy CR et al.(1999) Salt-sensitivity hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. Nat. Med. 5:217-220.
    Reference 2: 2. Narumiya S and FitzGerald GA (2001) Genetic and pharmacological analysis of prostanoid receptor function. J. Clin. Invest. 108: 25-30.
    Reference 3: 3. Yang N et al. (2003) Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor. J. Clin. Invest. 111: 727–735.