HTS028RTA - Ready-to-Assay™ sst2 Somatostatin Receptor Frozen Cells

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    Somatostatin (sst) is a multifunctional peptide with two biologically active forms, sst-14 and sst-28, which are synthesized in neurons throughout the brain as well as in peripheral tissues such as the pancreas and the gut (Gillies, 1997). SST exerts a diverse array of effects that include inhibition of endocrine secretion, modulation of neurotransmission, and regulation of cell proliferation by stimulating a family of five G-protein-coupled receptors. Somatostatin receptor sst2 mRNA is predominantly expressed in central nervous system. Study using sst2 knock-out mice has found the increased anxiety-related behaviour while locomotor and exploratory activity was decreased in stress-inducing situations (coupled with an increase in pituitary ACTH release, a regulator of the stress response) (Viollet et al., 2000). In the periphery, inhibition of glucagon release by sst in mouse islets is primarily mediated via sst2 (Strowski et al., 2000).  In addition, endogenous sst functions through sst2 to suppress gastric acid secretion through inhibition of gastrin activity (Martinez et al., 1998). Cloned human sst2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at sst2.

    Additional Resource: HTS028RTA080514_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: NM_001050.2
    Protein Targets: sst2
    Target Sub-family: Somatostatin
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: SSTR2 cDNA (Accession Number: NM_001050.2; see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Gillies G (1997) Somatostatin: the neuroendocrine story. Trends Pharmacol. Sci. 18: 87-95.
    Reference 2: 2. Martinez V et al. (1998) High basal gastric acid secretion in somatostatin receptor subtype 2 knockout mice. Gastroenterology 114: 1125 – 1132.
    Reference 3: 3. Strowski MZ et al. (2000) Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice. Endocrinology 141: 111 – 117.
    Reference 4: 4. Viollet C et al. (2000) Involvement of sst2 somatostatin receptor in locomotor, exploratory activity and emotional reactivity in mice Eur. J. Neurosci. 12: 3761 – 3770.