Calcium Flux Assay

HTS025RTA - Ready-to-Assay™ MCH1 Melanin-Concentrating Hormone Receptor Frozen Cells

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    [Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992). When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001). A receptor for MCH, previously identified as an orphan receptor SLC-1 and renamed MCH1, has been identified in humans and rodents.  Consistent with a role in hypothalamic control of feeding behavior, MCH1 is expressed in in the ventromedial and dorsomedial nuclei of the hypothalamus (Chambers et al., 1999).  Genetic ablation and pharmacological antagonism of MCH1 in rodents results in resistance to obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002;  Segal-Lieberman, et al., 2003;  Shearman et al., 2003;  Marsh et al., 2002;  Chen et al., 2002). Thus, MCH1 is an attractive target for obesity. Cloned human MCH1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCH1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MCH1.

    Additional Resource: HTS025RTA092714_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: NM_005297
    Protein Targets: MCH1
    Target Sub-family: Melanin
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: MCHR1 cDNA (Accession Number: NM_005297; see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Bittencourt J.C., et al. (1992) The melanin-concentrating hormone system of the rat brain: an immuno- and hybridization histochemical characterization. J. Comp. Neurol. 319: 218-45.
    Reference 2: 2. Borowsky B., et al. (2002) Antidepressant, anxiolytic and anorectic effects of a melanin-concentrating hormone-1 receptor antagonist. Nat. Med. 8: 825-30.
    Reference 3: 3. Chambers J., et al. (1999) Melanin-concentrating hormone is the cognate ligand for the orphan G-protein-coupled receptor SLC-1. Nature 400: 261-5.
    Reference 4: 4. Chen Y., et al. (2002) Targeted disruption of the melanin-concentrating hormone receptor-1 results in hyperphagia and resistance to diet-induced obesity. Endocrinology 143: 2469-77.
    Reference 5: 5. Ludwig D.S., et al. (2001) Melanin-concentrating hormone overexpression in transgenic mice leads to obesity and insulin resistance. J. Clin. Invest. 107: transgenic mice leads to obesity and insulin resistance. J. Clin. Invest. 107: 37
    Reference 6: 6. Marsh D.J., et al. (2002) Melanin-concentrating hormone 1 receptor-deficient mice are lean, hyperactive, and hyperphagic and have altered metabolism. Proc. Natl. Acad. Sci. USA 99: 3240-5.