Calcium Flux Assay

HTS016RTA - Ready-to-Assay™ C3a Anaphylotoxin Receptor Frozen Cells

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    CX3CR1 is a GPCR expressed on natural killer cells, cytotoxic T lymphocytes, and macrophages.  The sole ligand for CX3CR1, fractalkine, is an unusual chemokine that is expressed as a transmembrane molecule with a CX3C domain and a mucin domain (Imai et al., 1997).  Fractalkine is highly expressed on endothelial cells activated by TNFα and other proinflammatory cytokines, and fractalkine/CX3CR1 interactions mediate recruitment of macrophages into the atherosclerotic plaque (Lesnick et al., 2003; McDermott et al., 2003).  In addition, fractalkine and CX3CR1 have been implicated in the pathogenesis of glomerulonephritis, HIV infection and rheumatoid arthritis (Ito et al., 2002; Faure et al., 2003; Nanki et al., 2002). Cloned human CX3CR1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CX3CR1 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CX3CR1.

    Additional Resource: HTS016RTA092614_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: U28488
    Protein Targets: C3a
    Target Sub-family: Anaphylotoxin
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: Human C3AR1 cDNA (Accession Number: U28488) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Ember, JA and Hugli, TE (1997) Complement factors and their receptors. Immunopharmacology 38: 3-15.
    Reference 2: 2. Martin, U, et al. (1997) The human C3a receptor is expressed on neutrophils and monocytes, but not on B or T lymphocytes. J. Exp. Med. 186: 199-207.
    Reference 3: 3. Crass T, et al. (1996) Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells. Eur. J. Immunol. 26: 1944-50.
    Reference 4: 4. Humbles AA, et al. (2000) A role for the C3a anaphylatoxin receptor in the effector phase of asthma. Nature 406: 998-1001.
    Reference 5: 5. Drouin SM, et al. (2002) Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.J. Immunol. 169: 5926-33.
    Reference 6: 6. Ratajczak J, et al. (2004) Mobilization studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow. Blood 103: 2071-8.