Calcium Flux Assay

HTS014RTA - Ready-to-Assay™ CCR10 Chemokine Receptor Frozen Cells

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    CCR8 is a GPCR that binds primarily to the chemokine I-309 (CCL1) (Roos et al., 1997).  CCR8 is expressed primarily on Th2 cells although its functional role in T cell recruitment to sites of allergic inflammation is controversial (Chensue et al., 2001; Goya et al., 2003).  CCR8+ T-cells are abundant in skin, and rare or absent in the GI tract and peripheral blood, indicating a role in skin-homing of T-cells (Schaerli et al., 2004).  In addition, monocyte-derived dendritic cells express CCR8 and utilize CCR8 during mobilization from skin to the lymph node  (Qu et al., 2004). Cloned human CCR8-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR8 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR8.

    Additional Resource: HTS014RTA092314_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: AF215981
    Protein Targets: CCR10
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: CCR10 cDNA (Accession Number: AF215981) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Bertini R et al. (2004) Noncompetitive allosteric inhibitors of the inflammatory chemokine receptors CXCR1 and CXCR2: prevention of reperfusion injury. Proc. Natl. Acad. Sci. U S A.
    Reference 2: 2. Cummings CJ et al. (1999) Expression and function of the chemokine receptors CXCR1 and CXCR2 in sepsis. J. Immunol. 162(4):2341-6.
    Reference 3: 3. Hall DA et al. (1999) Signalling by CXC-chemokine receptors 1 and 2 expressed in CHO cells: A comparison of calcium mobilization, inhibition of adenylyl cyclase and stimulation of
    Reference 4: 4. Kaneider NC et al. (2005) Reversing systemic inflammatory response syndrome with chemokine receptor pepducins. Nat. Med. 11(6):661-5.
    Reference 5: 5. Murphy PM (1997) Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin. Hematol. 34: 311-8.
    Reference 6: 6. Sturm A et al. (2005) CXCL8 modulates human intestinal epithelial cells through a CXCR1 dependent pathway. Cytokine 29(1):42-8.