HTS012RTA - Ready-to-Assay™ CCR7 Chemokine Receptor Frozen Cells

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    CCR7 is a GPCR that binds to CC chemokine ligands MIP-3β (ELC/CCL19) and 6Ckine (CCL21) (Yoshiida et al., 1997). These ligands are expressed in the secondary lymphoid organs, and binding to CCR7 expressed in naïve T cells, B cells and dendritic cells directs migration of these cells to sites of antigen presentation (Forster et al., 199(. Inhibition of CCR7/ligand interactions inhibits contact sensitivity, delayed type hypersensitivity, and graft vs host disease in experimental models (Forster et al., 1999; Sasaki et al., 2003). In addition, CCR7 expression by breast cancer, melanoma, and other malignant cells are associated with lymph node metastasis (Muller et al., 2001; Payne and Cornelius, 2002). Cloned human CCR7-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR7 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR7.

    Additional Resource: HTS012RTA092614_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: L08176
    Protein Targets: CCR7
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: CCR7 cDNA (Accession Number: L08176).
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Förster R et al. (1999) CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell 99: 23-33.
    Reference 2: 2. Muller A et al. (2001) Involvement of chemokine receptors in breast cancer metastasis. Nature 410: 50-6.
    Reference 3: 3. Payne AS and Cornelius LA (2002) The role of chemokines in melanoma tumor growth and metastasis. J. Invest. Dermatol. 118: 915-22.
    Reference 4: 4. Sasaki M et al. (2003) Antagonist of secondary lymphoid-tissue chemokine (CCR ligand 21) prevents the development of chronic graft-versus-host disease in mice. J. Immunol. 170: 588-96.
    Reference 5: 5. Yoshida, R, et al. (1997) Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7. J. Biol. Chem. 272: 13803-9.