Calcium Flux Assay

HTS011RTA - Ready-to-Assay™ CCR6 Chemokine Family Receptor Frozen Cells

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    CCR6 is a GPCR that binds to a single chemokine, CCL20 (also known as MIP-3α, LARC or Exods-1), as well as several members of the beta-defensin family of antimicrobial peptides (Schutyser, 2003).  Immature dendritic cells, B-cells, and effector/memory T-cells express CCR6, and the receptor promotes inflammation during the development of rheumatoid arthritis, inflammatory bowel disease, and graft-versus-host disease (Varona et al., 2003; Kleinewietfeld et al., 2005; Varona et al., 2005).  Cloned human CCR6-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR6 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR6.

    Additional Resource: HTS011RTA092214_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: U45984
    Protein Targets: CCR6
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: CCR6 cDNA (Accession Number: U45984; see CODING SEQUENCE below) expressed from a proprietary pHS plasmid
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Kleinewietfeld M et al. (2005) CCR6 expression defines regulatory effector/memory-like cells within the CD25(+)CD4+ T-cell subset. Blood 105: 2877-86.
    Reference 2: 2. Schutyser E (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14: 409-26.
    Reference 3: 3. Varona R et al. (2003) CCR6 has a non-redundant role in the development of inflammatory bowel disease. Eur. J. Immunol. 33: 2937-46.
    Reference 4: 4. Varona R et al. (2005) CCR6 regulates CD4+ T-cell-mediated acute graft-versus-host disease responses. Blood 106: 18-26.