HTS011M - ChemiScreen™ CCR6 Chemokine Membrane Preparation

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    CCR6 is a GPCR that binds to a single chemokine, CCL20 (also known as MIP-3a, LARC or Exodus-1), as well as several members of the beta-defensin family of antimicrobial peptides (Schutyser, 2003).  Immature dendritic cells, B-cells, and effector/memory T-cells express CCR6, and the receptor promotes inflammation during the development of rheumatoid arthritis, inflammatory bowel disease, and graft-versus-host disease (Varona et al., 2003; Kleinewietfeld et al., 2005; Varona et al., 2005).  CCR6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR6 interactions with MIP-3a.  The membrane preparations exhibit a Kd of 0.09 nM for [125I]-MIP-3a.  With 0.3 nM [125I]-MIP-3a, 5 ug/well and 10 ug/well CCR6 Membrane Prep typically yield greater than 6-fold and 8-fold signal-to-background ratios, respectively.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: Radioligand Binding Assay
    Entrez Gene Number: NM_004367.5, NM_031409.3
    Protein Targets: CCR6
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous CCR6 expression.
    Reference 1: 1. Kleinewietfeld M et al. (2005) CCR6 expression defines regulatory effector/memory-like cells within the CD25(+)CD4+ T-cell subset. Blood 105: 2877-86.
    Reference 2: 2. Schutyser E (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14: 409-26.
    Reference 3: 3. Varona R et al. (2003) CCR6 has a non-redundant role in the development of inflammatory bowel disease. Eur. J. Immunol. 33: 2937-46.
    Reference 4: 4. Varona R et al. (2005) CCR6 regulates CD4+ T-cell-mediated acute graft-versus-host disease responses. Blood 106: 18-26.