HTS009RTA - Ready-to-Assay™ CCR4 Chemokine Receptor Frozen Cells

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    CCR4 is a GPCR that is activated by chemokines TARC (thymus and activation regulated chemokine) and MDC (macrophage-derived chemokine) (Olsen and Ley, 2001).  NK cells, Th2 cells, cutaneous memory T cells, macrophages and platelets express CCR4 and respond to TARC and MDC (Andrew et al., 2001; Inngjerdingen et al., 2000; Gear et al., 2001; Soler et al., 2003). CCR4-null mice display reduced airway hyperresponsiveness to fungal spores, lipopolysaccharide-induced endotoxic shock, and cardiac allograft rejection (Chvatchko et al., 2000; Schuh et al., 2002; Huser et al., 2005).  In addition, antibody –mediated antagonism of MDC inhibits development of insulitis and diabetes in NOD mice (Kim et al., 2002).  Cloned human CCR4 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CCR4 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR4.

    Additional Resource: HTS009RTA082714_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: X85740
    Protein Targets: CCR4
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-5, an adherent rat hematopoietic cell line expressing endogenous Ga15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exogenous Gene Expression: CCR4 cDNA (Accession Number: X85740) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Andrew DP et al., (2001) C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential. J. Immunol. 166: 103-11
    Reference 2: 2. Chvatchko Y et al. (2000) A key role for CC chemokine receptor 4 in lipopolysaccharide-induced endotoxic shock. J. Exp. Med. 191: 1755-64.
    Reference 3: 3. Gear AR et al. (2001) Adenosine diphosphate strongly potentiates the ability of the chemokines MDC, TARC, and SDF-1 to stimulate platelet function. Blood 97: 937-45
    Reference 4: 4. Huser N et al. (2005) CCR4-deficient mice show prolonged graft survival in a chronic cardiac transplant rejection model. Eur. J. Immunol. 35: 128-138.
    Reference 5: 5. Inngjerdingen M et al. (2000) Human NK cells express CC chemokine receptors 4 and 8 and respond to thymus and activation-regulated chemokine, macrophage-derived chemokine, and I-309. J. Immunol. 164: 4048-54
    Reference 6: 6. Kim SH et al. (2002) CCR4-bearing T cells participate in autoimmune diabetes. J. Clin. Invest. 110: 1675-1686.