Calcium Flux Assay

HTS008RTA - Ready-to-Assay™ CCR3 Chemokine Receptor Frozen Cells

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    Eosinophils are major effector cells implicated in a number of chronic inflammatory diseases in humans, particularly bronchial asthma and allergic rhinitis. The chemokine receptor 3 (CCR3), a GPCR activated by chemokines eotaxin 1/2, MCP-3, MCP-4, and RANTES, mediates selective recruitment of eosinophils into tissue and thus has recently become an attractive biological target for therapeutic intervention (Fujisawa et al., 2000).  It is widely expressed on cells involved in allergic inflammation, such as basophils, mast cells, airway epithelial cells, and potentially TH2 T-lymphocytes.  Allergen-induced eosinophil infiltration into airways is reduced or eliminated in CCR3 and eotaxin 1/2 knockout mice mice and in mice treated with antibodies directed against CCR3 (Grimaldi et al., 1999;  Fulkerson et al., 2006). CCR3 antagonists are currently being developed for the treatment of asthma and other allergic disorders. Cloned human CCR3-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CCR3 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at CCR3.

    Additional Resource: HTS008RTA030615_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: U28694
    Protein Targets: CCR3
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-4, an adherent rat hematopoietic cell line expressing endogenous G15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exogenous Gene Expression: CCR3 cDNA (Accession Number: U28694) expressed from a proprietary plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Fujisawa T et al. (2000) Chemokines induce eosinophil degranulation through CCR-3. J. Allergy Clin. Immunol. 106: 507–513.
    Reference 2: 2. Fulkerson PC et al. (2006) A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc. Natl. Acad. Sci. USA 103: 16418-16423.
    Reference 3: 3. Grimaldi JC et al. (1999) Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3). J. Leukoc. Biol. 65: 846–853.