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HTS003L - ChemiBrite™ CXCR3 Chemokine Receptor Stable Cell Line

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    CXCR3 is a 7-TM GPCR that is selective for the CXC chemokines IP10, I-TAC and MIG (Loetscher et al., 1996). Binding of IP10 and MIG to CXCR3 induces Ca2+ mobilization, chemotaxis and inflammatory responses of T lymphocytes, and also act as potent inhibitors of angiogenesis. CXCR3 is highly expressed in IL-2-activated T lymphocytes in vitro (Loetscher et al., 1996), and in T lymphocytes present in inflamed tissues in rheumatoid arthritis and multiple sclerosis (Balashoy et al., 1999; Qin et al., 1998). In vivo, neutralization of CXCR3 inhibits experimentally induced type I diabetes (Frigerio et al., 2002), peritonitis (Xie et al., 2003), and post-lung transplantation bronchiolitis obliterans syndrome (Belperio et al., 2002). Eurofins' cloned human CXCR3 receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin, the receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. These stability-tested cells are ready for luminescent analysis of agonists, antagonists and modulators at the CXCR3 receptor.

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    Item Unit of Measure: pk
    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: NM_001504.1
    Host Cell: HEK293
    Exogenous Gene Expression: Human CXCR3 cDNA (Accession Number: NM_001504.1; see CODING SEQUENCE below) and a proprietary mutant
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Loetscher M, et al. (1996) Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.J. Exp. Med. 184(3): 963-9.
    Reference 2: 2. Balashov, KE, et al. (1999) CCR5 (+) and CXCR3 (+) T cells are increased in multiple sclerosis and their ligands MIP-1alpha and IP-10 are expressed in demyelinating brain lesions.Proc. Natl. Acad. Sci. USA 96: 6873-8.
    Reference 3: 3. Qin, S, et al. (1998) The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions.J. Clin. Invest. 101: 746-54.
    Reference 4: 4. Frigerio, S, et al. (2002) Beta cells are responsible for CXCR3-mediated T-cell infiltration in insulitis. Nat. Med. 8: 1414-20.
    Reference 5: 5. Xie, JH, et al. (2003) Antibody-mediated blockade of the CXCR3 chemokine receptor results in diminished recruitment of T helper 1 cells into sites of inflammation.J. Leukoc. Biol. 73: 771-7-80.
    Reference 6: 6. Belperio, JA, et al. (2002) Critical role for CXCR3 chemokine biology in the pathogenesis of bronchiolitis obliterans syndrome. J. Immunol. 169: 1037-1049.