Calcium flux assay

HTS002RTA - Ready-to-Assay™ CXCR2 Chemokine Receptor Frozen Cells

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    CXCR2 is a 7-TM G-protein coupled receptor that binds to the chemokines GROα, GROβ, GROγ, IL-8, ENA-78, NAP-2 and GCP-2 (Olson and Ley, 2002). Neutrophils, mast cells and microvascular endothelial cells express CXCR2, and ligand interactions with CXCR2 promote chemotaxis of these cell types (Heidemann et al., 2003; Nilsson et al., 1999; White et al., 1998). Studies with mice lacking CXCR2 indicate that CXCR2 promotes growth of primary tumors and secondary metastases (Keane et al., 2004), and plays an essential role in hyperoxia-induced lung injury (Sue et al., 2004). In addition, cytomegalovirus encodes a CXCR2-binding chemokine, vCXC-1, that promotes neutrophil migration to infected cells (Penfold et al., 1999). The cloned human CXCR2 ]-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CXCR2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR2.

    Additional Resource: HTS002RTA092214_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: M73969
    Protein Targets: CXCR2 / IL-8b
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein
    Exogenous Gene Expression: IL8RA cDNA (Accession Number: M68932) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Heidemann J, et al. (2003) Angiogenic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2. J. Biol. Chem. 278: 8508-15
    Reference 2: 2. Keane MP, et al. (2004) Depletion of CXCR2 inhibits tumor growth and angiogenesis in a murine model of lung cancer. J. Immunol. 172: 2853-60.
    Reference 3: 3. Nilsson G, et al. (1999) Mast cell migratory response to interleukin-8 is mediated through interaction with chemokine receptor CXCR2/Interleukin-8RB. Blood 93: 2791-7
    Reference 4: 4. Olson TS and Ley K (2002) Chemokines and chemokine receptors in leukocyte trafficking. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R7-28
    Reference 5: 5. Penfold ME, et al. (1999) Cytomegalovirus encodes a potent alpha chemokine. Proc Natl Acad Sci USA 96: 9839-44
    Reference 6: 6. Sue RD, et al. (2004) CXCR2 is critical to hyperoxia-induced lung injury. J. Immunol. 172: 3860-8