HTS001RTA - Ready-to-Assay™ CXCR1 Chemokine Receptor Frozen Cells

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    CXCR1 (also known as IL-8RA) is a Gi/o-coupled GPCR expressed on neutrophils, CD8(+) T cells, and intestinal epithelial cells (Murphy, 1997; Sturm et al., 2005; Takata et al., 2004). CXCR1 binds with high affinity to the chemokine interleukin-8 (IL-8) and much lower affinity to GROα, whereas a related receptor, CXCR2, binds with equal affinity to IL-8 and GROα (Hall et al., 1999). CXCR1 has been proposed to be the primary regulator of neutrophil response to IL-8 in sepsis (Cummings et al., 1999). Antagonists of CXCR1 and CXCR2 have been developed and display efficacy in animal models of reperfusion injury (Bertini, et al., 2004; Kaneider et al., 2005). Chemicon's cloned human CXCR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CXCR1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CXCR1 and its ligands.

    Additional Resource: HTS001RTA092614_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: NM_000634.2
    Protein Targets: CXCR1 / IL-8a
    Target Sub-family: Chemokine
    Species: Human
    Host Cell: Host Cell
    Exogenous Gene Expression: IL8RA cDNA (Accession Number: M68932) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Bertini R et al. (2004) Noncompetitive allosteric inhibitors of the inflammatory chemokine receptors CXCR1 and CXCR2: prevention of reperfusion injury. Proc. Natl. Acad. Sci. U S A. 101(32):11791-6.
    Reference 2: 2. Cummings CJ et al. (1999) Expression and function of the chemokine receptors CXCR1 and CXCR2 in sepsis. J. Immunol. 162(4):2341-6.
    Reference 3: 3. Hall DA et al. (1999) Signalling by CXC-chemokine receptors 1 and 2 expressed in CHO cells: A comparison of calcium mobilization, inhibition of adenylyl cyclase and stimulation of GTP S binding induced by IL-8 and GROa. Br. J. Pharmacol. 126: 810-
    Reference 4: 4. Kaneider NC et al. (2005) Reversing systemic inflammatory response syndrome with chemokine receptor pepducins. Nat. Med. 11(6):661-5.
    Reference 5: 5. Murphy PM (1997) Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin. Hematol. 34: 311-8.
    Reference 6: 6. Sturm A et al. (2005) CXCL8 modulates human intestinal epithelial cells through a CXCR1 dependent pathway. Cytokine 29(1):42-8.