GTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS BindingGTPγS Binding

HTS036M - ChemiSCREEN™ CCR9 Chemokine Receptor Membrane Preparation

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    The CCR9 receptor is a GPCR expressed selectively in the thymus, lymph node, spleen, and the small bowel (SB). CCR9 binds to Thymus-expressed chemokine ligand (TECK / CCL25) (Zaballos et al. 1999; Youn et al., 1999; Yu et al., 2000), and is involved in directing the movement of mature T cells in the thymus (Carramolino et al., 2001b). CCR9 also plays an important role in the pathogenesis of SB Crohn’s disease (Carramolino et al., 2001a; Papadakis et al, 2001). The CCR9 selective expression in the small bowel but not the colon indicates the role of CCR9 in the specialized immune response in the SB (Kunkel et al., 2000; Papadakis et al., 2000). CCR9 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR9 interactions with its ligands. The membrane preparations exhibit an EC50 of 9 nM for TECK/CCL25 in a GTPgS binding assay.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial
    Applications: GTPγS Binding
    Species: Human CCR9 (Accession number NM_031200).
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous CCR9 expression.
    Reference 1: 1. Carramolino L, et al. (2001a) CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease. Gastroenterology. 121(2):246-54.
    Reference 2: 2. Carramolino L, et al. (2001b) Expression of CCR9 beta-chemokine receptor is modulated in thymocyte differentiation and is selectively maintained in CD8(+) T cells from secondary lymphoid organs. Blood. 15-97(4):850-7.
    Reference 3: 3. Kunkel EJ, et al. (2000) Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing princ
    Reference 4: 4. Papadakis KA, et al. (2000) The role of thymus-expressed chemokine and its receptor CCR9 on lymphocytes in the regional specialization of the mucosal immune system. J Immunol. 165(9):5069-76.
    Reference 5: 5. Papadakis KA, et al. (2001) CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease. Gastroenterology. 121(2):246-54.
    Reference 6: 6. Yu CR, et al. (2000) CCR9A and CCR9B: two receptors for the chemokine CCL25/TECK/Ck beta-15 that differ in their sensitivities to ligand. J Immunol. 1-164(3):1293-305.
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