HTS030M - ChemiSCREEN™ PTH Receptor Membrane Preparation

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$646.90
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HTS030M
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    Parathyroid hormone (PTH) plays a critical role in mineral ion homeostasis, particularly in bone and kidney (Mannstadt et al., 1999; Gensure et al., 2005). A related peptide, parathyroid hormone-related peptide (PTHrP), plays an important role in skeletal development. Both peptides exert their biological actions by binding to a class B GPCR, PTH1R, that signals primarily by activation of adenylate cyclase (Schipani et al., 1993). Mutations in PTH1R have been identified as the cause of Jansen's metaphyseal chondrodysplasia, Blomstrand's chondrodysplasia, and enchondromatosis (Schipani and Provot, 2003). Intermittant treatment with PTH has important clinical utility in building bone mass in patients with osteoporosis (Rosen, 2003). Cloned human PTH1R- membrane preparation is an ideal tool for screening for interactions between PTH1R and its ligands.

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial
    Applications: Radioligand Binding Assay
    Species: Full-length human PTH1R cDNA (Accession Number: NM_000316)
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous PTH expression.
    Reference 1: 1. Gensure RC et al. (2005) Parathyroid hormone and parathyroid hormone-related peptide, and their receptors. Biochem. Biophys. Res. Commun. 328: 666-78.
    Reference 2: 2. Mannstadt M et al. (1999) Receptors for PTH and PTHrP: their biological importance and functional properties. Am. J. Physiol. 277(5 Pt 2): F665-75.
    Reference 3: 3. Rosen CJ (2003) The cellular and clinical parameters of anabolic therapy for osteoporosis. Crit. Rev. Eukaryot. Gene Expr. 13: 25-38.
    Reference 4: 4. Schipani E and Provot S. (2003) PTHrP, PTH, and the PTH/PTHrP receptor in endochondral bone development. Birth Defects Res. C. Embryo Today 69: 352-62.
    Reference 5: 5. Schipani E (1993) Identical complementary deoxyribonucleic acids encode a human renal and bone parathyroid hormone (PTH)/PTH-related peptide receptor. Endocrinology 132: 2157-65.
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